Tabersonine hydrochloride

Catalog No.S5101 Batch:S510101

Technical Data

Formula	C₂₁H₂₄N₂O₂.HCl
Molecular Weight	372.89	CAS No.	29479-00-3
Solubility (25°C)*	In vitro	DMSO	40 mg/mL (107.27 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Tabersonine, an ingredient extracted from the bean of Voacanga africana, is a potent inhibitor against Aβ(1−42) aggregation and toxicity.

Targets

Aβ(1−42) aggregation ^[1]

In vitro

Tabersonine disrupts Aβ(1−42) aggregation and ameliorates Aβ aggregate-induced cytotoxicity. It binds to Aβ(1−42) oligomers more strongly than to Aβ(1−42) monomers with Kd values of 69 μM and 535 μM, respectively^[1].

In vivo

The biocompatibility and small size essential for permeating the blood−brain barrier make tabersonine a potential therapeutic drug candidate for treating Alzheimer's disease (AD)^[1].

Protocol (from reference)

Cell Assay:^[1]	Cell lines SH-SY5Y cell Concentrations 10 μM Incubation Time 24 h Method The cultured cells were transferred to a sterile 96-well plate with approximately 1×104 cells/well. Cells acclimatized overnight in the media in a humidified incubator under 5% CO2 at 37℃. Aβ(1−42)solutions and Aβ(1−42)−tabersonine mixtures were preincubated at 37℃ for different times, and the resultant solutions were mixed with the SH-SY5Y cells for 24 h. The viability of the SH-SY5Y cells exposed to each solution was determined with MTT assay. Cell viability was estimated by dividing the absorbance of wells containing samples by that of wells containing only cell culture media.

Cell Assay:^[1]

Cell lines
SH-SY5Y cell
Concentrations
10 μM
Incubation Time
24 h
Method
The cultured cells were transferred to a sterile 96-well plate with approximately 1×104 cells/well. Cells acclimatized overnight in the media in a humidified incubator under 5% CO2 at 37℃. Aβ(1−42)solutions and Aβ(1−42)−tabersonine mixtures were preincubated at 37℃ for different times, and the resultant solutions were mixed with the SH-SY5Y cells for 24 h. The viability of the SH-SY5Y cells exposed to each solution was determined with MTT assay. Cell viability was estimated by dividing the absorbance of wells containing samples by that of wells containing only cell culture media.

References

[1] Kai T, et al. ACS Chem Neurosci. 2015, ;6(6):879-88.

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SHIPPING AND STORAGE
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