SodiuM Metatungstate

Catalog No.S5525 Batch:S552501

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Technical Data

Formula

H2O40W12.6Na
Molecular Weight 2986.01 CAS No. 12141-67-2
Solubility (25°C)* In vitro Water 50 mg/mL (16.74 mM)
DMSO 30 mg/mL (10.04 mM)
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Sodium metatungstate (SMT) solution is an inorganic heavy liquid which is widely used in density fractionation. Sodium metatungstate (Sodium polyoxotungstates) is a potent and selective inhibitor of ecto-nucleoside triphosphate diphosphohydrolase (NTPDases, CD39) with Ki of 0.140 μM, 0.910 μM and 0.563 μM for NTPDase1, NTPDase2 and NTPDase3, respectively.
Targets
NTPDase1 [1]
(Cell-free assay)		 NTPDase3 [1]
(Cell-free assay)		 NTPDase2 [1]
(Cell-free assay)
0.140 μM(Ki) 0.563 μM(Ki) 0.910 μM(Ki)
In vitro

Sodium metatungstate, a inhibitor of CD39, can partially reverse the suppression of CD8[+H] T cells induced by the presence of peritoneal macrophage.[2]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Peritoneal lavage cells, CD8+ T cells (purified from ACK-lysed splenocytes from C57BL/6 mice)

  • Concentrations

    50 μM

  • Incubation Time

    48 h

  • Method

    Peritoneal lavage cells are plated in complete RPMI media on a 10 cm dish. Celexocib (10 μM), anti-TGF-b (clone 1D11, 10 μg/ml), or sodium metatungstate (50 μM) are plated onto respective wells of a 96 well round bottom plate. After at least five rounds of gentle aspiration and washing of the 10 cm plate containing the peritoneal lavage cells, adherent peritoneal macrophages are removed with a cell scraper, collected, and counted. 105 peritoneal macrophages are plated per indicated well of the 96 well plates. CD8+ T cells are purified from ACK-lysed splenocytes from C57BL/6 mice utilizing the Mouse CD8a+ T Cell Isolation Kit and then 105 purified CD8+ T cells are plated onto in T cell medium with 50 IU/ml murine IL-2. Certain wells are incubated with 105 anti-mouse CD3/CD28 Dynabeads for T cell activation. Flow cytometry is performed after 48 hours of culture.

Selleck's SodiuM Metatungstate has been cited by 2 publications

CD39 pathway inhibits Th1 cell function in tuberculosis [ Immunology, 2022, 10.1111/imm.13493] PubMed: 35574713
Tim-4+ cavity-resident macrophages impair anti-tumor CD8+ T cell immunity [ Cancer Cell, 2021, 39(7):973-988.e9] PubMed: 34115989

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.