Sarcosine

Catalog No.S4702 Batch:S470201

Technical Data

Formula	C₃H₇NO₂
Molecular Weight	89.09	CAS No.	107-97-1
Solubility (25°C)*	In vitro	Water	17 mg/mL (190.81 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Sarcosine (N-methylglycine, Sarcosinic acid, Methylaminoacetic acid, Methylglycine) is a competitive inhibitor of the type I glycine transporter (GlyT1) and an N-methyl-D-aspartate receptor (NMDAR) co-agonist.

Targets

GlyT1 ^[1]

N-methyl-D-aspartate receptor ^[1]

GlyR ^[1]

In vitro

Sarcosine is a GlyR agonist in addition to being a GlyT1 inhibitor and NMDAR co-agonist, but it is less potent than glycine as a GlyR agonist and is not a full agonist^[1]. The viability of the sarcosine-treated cells is significantly reduced^[4].

In vivo

Sarcosine has weak anticonvulsant properties^[2]. It ameliorates (prepulse inhibition)PPI deficits in mGluR5 knockout mice^[3].

Protocol (from reference)

Cell Assay:^{^[4]}	Cell lines PC-3 prostate cancer cell line Concentrations 10, 150, 250, 500, 1,000 and 1,500 μM Incubation Time 0, 6, 12, 24 and 72 h Method Immediately after the cells grow to 50-60% confluence, the cultivation medium is replaced by fresh medium to synchronise cell growth. Cells are cultivated for 24 h under these conditions. Subsequently, the culture medium is supplemented with sarcosine (N-methylglycine) diluted to a final concentration 10, 150, 250, 500, 1,000 and 1,500 μM. Treatment is carried out for 0, 6, 12, 24 and 72 h, and samples are collected at these strictly defined time points.
Animal Study:^{^[2]}	Animal Models Male Albino Swiss mice Dosages 100,200,400 and 800 mg/kg Administration i.p.

Cell Assay:^{^[4]}

Cell lines
PC-3 prostate cancer cell line
Concentrations
10, 150, 250, 500, 1,000 and 1,500 μM
Incubation Time
0, 6, 12, 24 and 72 h
Method
Immediately after the cells grow to 50-60% confluence, the cultivation medium is replaced by fresh medium to synchronise cell growth. Cells are cultivated for 24 h under these conditions. Subsequently, the culture medium is supplemented with sarcosine (N-methylglycine) diluted to a final concentration 10, 150, 250, 500, 1,000 and 1,500 μM. Treatment is carried out for 0, 6, 12, 24 and 72 h, and samples are collected at these strictly defined time points.

Animal Study:^{^[2]}

Animal Models
Male Albino Swiss mice
Dosages
100,200,400 and 800 mg/kg
Administration
i.p.

References

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SHIPPING AND STORAGE
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