RSL3

Catalog No.S8155 Batch:S815502

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Technical Data

Formula

C23H21ClN2O5
Molecular Weight 440.88 CAS No. 1219810-16-8
Solubility (25°C)* In vitro DMSO 88 mg/mL (199.6 mM)
Ethanol 28 mg/mL (63.5 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description RSL3 ((1S,3R)-RSL3) is a ferroptosis activator in a VDAC-independent manner,exhibiting selectivity for tumor cells bearing oncogenic RAS. RSL3 binds, inactivates GPX4 and thus mediates GPX4-regulated ferroptosis.
Targets
Gpx4 [1]
(In Calu-1 cells)
In vitro Ferroptosis-inducing compounds inactivate GPX4 by direct binding or by depleting glutathione.After binding to GPX4, RSL3 inactivates GPX4 to induce ROS production from lipid peroxidation. RSL3's ability to induce synthetic lethality with oncogenic RAS is rapid and quite potent. This compound inhibits the growth of BJ-TERT/LT/ST/RASV12 and DRD cells as low as 10 ng/mland started to kill sensitive cells as early as 8 hr after treatment[1][2].
In vivo Prostaglandin-endoperoxide synthase (PTGS) is the key enzyme in prostaglandin biosynthesis. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2. PTGS2 encoding cyclooxygenase-2 (COX-2) is significantly upregulated after treatment with RSL3 and erastin in mice[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    TERT/LT/ST/RASV12 cells 

  • Concentrations

    1 µg/ml

  • Incubation Time

    16hrs

  • Method

    TERT/LT/ST/RASV12 cells are seeded in 10 cm dishes and treated with 1 µM staurosporine, 10 µg/ml erastin, 20 µg/ml RSL5, and 1 µg/ml RSL3 for 16 hr. Both dying cells and live cells in each 10 cm dish are harvested and collected in the same 15 ml tubes by centrifuging cell suspension at 1000 rpm for 5 min.
Animal Study:

[1]
  • Animal Models

    athymic nude mice(8 weeks)implanted with subcutaneous xenograft tumors derived from BJeLR cells

  • Dosages

    100 mg/kg

  • Administration

    s.c. injection

Customer Product Validation

, , Am J Physiol Renal Physiol, 2017, doi: 10.1152/ajprenal.00044.2017

Data from [Data independently produced by , , Nature, 2017, 547(7664):453-457]

Data from [Data independently produced by , , Cell, 2017, 171(3):628-641]

Data from [Data independently produced by , , Cell Res, 2016, 26(9):1021-32]

Selleck's RSL3 has been cited by 343 publications

7-Dehydrocholesterol dictates ferroptosis sensitivity [ Nature, 2024, 626(7998):411-418.] PubMed: 38297130
Integration of 3D bioprinting and multi-algorithm machine learning identified glioma susceptibilities and microenvironment characteristics [ Cell Discov, 2024, 10(1):39] PubMed: 38594259
Pro-ferroptotic signaling promotes arterial aging via vascular smooth muscle cell senescence [ Nat Commun, 2024, 15(1):1429] PubMed: 38365899
Targeting of vulnerabilities of drug-tolerant persisters identified through functional genetics delays tumor relapse [ Cell Rep Med, 2024, 5(3):101471] PubMed: 38508142
Meningioma achieves malignancy and erastin-induced ferroptosis resistance through FOXM1-AURKA-NRF2 axis [ Redox Biol, 2024, 72:103137] PubMed: 38642502
Caspase-2 protects against ferroptotic cell death [ Cell Death Dis, 2024, 15(3):182] PubMed: 38429264
Dibutyl phthalate exposure induced mitochondria-dependent ferroptosis by enhancing VDAC2 in zebrafish ZF4 cells [ Environ Pollut, 2024, 348:123846] PubMed: 38548160
Novel SK channel positive modulators prevent ferroptosis and excitotoxicity in neuronal cells [ Biomed Pharmacother, 2024, 171:116163] PubMed: 38242037
Coculture with macrophages alters ferroptosis susceptibility of triple-negative cancer cells [ Cell Death Discov, 2024, 10(1):108] PubMed: 38429255
PKCiota Inhibits the Ferroptosis of Esophageal Cancer Cells via Suppressing USP14-Mediated Autophagic Degradation of GPX4 [ Antioxidants (Basel), 2024, 13(1)114] PubMed: 38247539

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.