Rafoxanide

Catalog No.S5093 Batch:S509301

Technical Data

Formula	C₁₉H₁₁Cl₂I₂NO₃
Molecular Weight	626.01	CAS No.	22662-39-1
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (159.74 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Rafoxanide (Disalan, Ranide, Flukanide, Bovanide) is a salicylanilide used as an anthelmintic, being effective treatment for the control of fluke infections in animals.
In vitro	Rafoxanide is a novel allosteric inhibitor of SPAK and OSR1. In vitro kinase assays using Rafoxanide showed it to be a low micromolar inhibitor of OSR1 T185E in vitro, IC50 = 8.18 μM. It is also a low micromolar inhibitor of SPAK T233E in vitro (IC50 = 13.03 μM)^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HEK293 cells Concentrations 1, 3, 10, 20, 50 μM Incubation Time 30 min Method HEK293 cells were first treated with Rafoxanide or Closantel at the indicated concentrations or STOCK1S5 0699 (10 μM) for 30 min and then either left untreated or treated with hypotonic buffer for 30 min to activate WNK-SPAK/OSR1 signalling. The cells were then harvested, and the lysates were probed for phospho-NKCC1 T203, T207 and T212, total NKCC1, phospho-SPAKS373, total SPAK and GAPDH as a loading control.

Cell Assay:^{^[1]}

Cell lines
HEK293 cells
Concentrations
1, 3, 10, 20, 50 μM
Incubation Time
30 min
Method
HEK293 cells were first treated with Rafoxanide or Closantel at the indicated concentrations or STOCK1S5 0699 (10 μM) for 30 min and then either left untreated or treated with hypotonic buffer for 30 min to activate WNK-SPAK/OSR1 signalling. The cells were then harvested, and the lysates were probed for phospho-NKCC1 T203, T207 and T212, total NKCC1, phospho-SPAKS373, total SPAK and GAPDH as a loading control.

References

[1] AlAmri MA, et al. ChemMedChem. 2017, 12(9):639-645.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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