Pyridostatin Trifluoroacetate Salt

Catalog No.S7444 Batch:S744402

Technical Data

Formula	C₃₁H₃₂N₈O₅·C₂HF₃O₂
Molecular Weight	710.7	CAS No.	1472611-44-1
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (140.7 mM)
		Water	100 mg/mL (140.7 mM)
		Ethanol	13 mg/mL (18.29 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Pyridostatin Trifluoroacetate Salt is a G-quadruplexe stabilizer with K_d of 490 nM in a cell-free assay, which targets a series of proto-oncogenes.

Targets

G-quadruplexe ^[1]
(Cell-free assay)

490 nM(Kd)

In vitro

Pyridostatin decreases the proliferation of MRC-5–SV40 cells and various cancer cell lines, and induces cell-cycle arrest by DNA-damage checkpoint activation. Pyridostatin also reduces SRC-dependent cell motility in MDA-MB-231 cells by interacting with G-quadruplex motifs in SRC. ^[2]

Pyridostatin decreases EBNA1 synthesis via inhibition of G-quadruplexes. ^[3]

In vivo

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines MRC-5–SV40 cells and various cancer cell lines Concentrations 10 μM Incubation Time 48 h Method Cells are plated at equal confluence and are left either untreated or were treated with 2 μM pyridostatin continually during the indicated time before harvesting the cells. Cells from individual plates are trypsinized and counted in a Coulter counter. Graphs represent the total cell numbers at each time interval, and the error bars represent the s.e.m. Data represent three independent experiments.
Animal Study:^{^[4]}	Animal Models CB17‐SCID mice Dosages 7.5 mg/kg Administration i.v.

Cell Assay:^{^[2]}

Cell lines
MRC-5–SV40 cells and various cancer cell lines
Concentrations
10 μM
Incubation Time
48 h
Method
Cells are plated at equal confluence and are left either untreated or were treated with 2 μM pyridostatin continually during the indicated time before harvesting the cells. Cells from individual plates are trypsinized and counted in a Coulter counter. Graphs represent the total cell numbers at each time interval, and the error bars represent the s.e.m. Data represent three independent experiments.

Animal Study:^{^[4]}

Animal Models
CB17‐SCID mice
Dosages
7.5 mg/kg
Administration
i.v.

References

[4] Groelly FJ, et al. EMBO Mol Med. 2022 Mar 7;14(3):e14501.

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Selleck's Pyridostatin Trifluoroacetate Salt has been cited by 9 publications

RAD54L2-mediated DNA damage avoidance pathway specifically preserves genome integrity in response to topoisomerase 2 poisons [ Sci Adv, 2023, 9(49):eadi6681]	PubMed: 38055811
G-quadruplex binders as cytostatic modulators of innate immune genes in cancer cells [ Nucleic Acids Res, 2021, gkab500]	PubMed: 34139015
Differential responses of neurons, astrocytes, and microglia to G-quadruplex stabilization [ Aging (Albany NY), 2021, 13(12):15917-15941]	PubMed: 34139671
Characterization of an active LINE-1 in the naked mole-rat genome [ Sci Rep, 2021, 11(1):5725]	PubMed: 33707548
hnRNP H/F Drive RNA G-quadruplex-mediated Translation Linked to Genomic Instability and Therapy Resistance in Glioblastoma [ Nat Commun, 2020, 27;11(1):2661]	PubMed: 32461552
Replication Stress Induces ATR/CHK1-Dependent Nonrandom Segregation of Damaged Chromosomes. [ Mol Cell, 2020, 22 pii: S1097-2765(20)30231-8]	PubMed: 32353258
Regulation of Autophagy by DNA G-quadruplexes [ Autophagy, 2020, 18]	PubMed: 32420812
HNRNPH1-dependent splicing of a fusion oncogene reveals a targetable RNA G-quadruplex interaction [ RNA, 2019, 25(12):1731-1750]	PubMed: 31511320
HNRNPH1-dependent splicing of a fusion oncogene reveals a targetable RNA G-quadruplex interaction [ RNA, 2019, 25(12):1731-1750]	PubMed: 31511320

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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