6-OHDA (Oxidopamine hydrobromide)

Catalog No.S5324 Batch:S532402

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Technical Data

Formula

C8H11NO3.HBr
Molecular Weight 250.09 CAS No. 636-00-0
Solubility (25°C)* In vitro DMSO 50 mg/mL (199.92 mM)
Water 50 mg/mL (199.92 mM)
Ethanol 13 mg/mL (51.98 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description 6-OHDA (Oxidopamine hydrobromide) is a neurotoxic synthetic organic compound that acts as an antagonist of the neurotransmitter dopamine with potential antineoplastic activity. Solutions are unstable and should be fresh-prepared.
In vitro

In PC12 cells, 6-hydroxydopamine (6-OHDA) results in the activation of caspases and apoptosis, also induces superoxide generation, Bid cleavage and mitochondrial membrane depolarization.In addition, Akt phosphorylation that is favorable to cell survival is decreased and p38 MAPK phosphorylation is increased by 6-OHDA.[1]
In vivo

Oxidopamine hydrobromide (6-OHDA hydrobromide), an antagonist of the neurotransmitter dopamine, is a widely used neurotoxin that selectively destroys dopaminergic neurons.[2]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    PC12 cells

  • Concentrations

    75 μM

  • Incubation Time

    12 h

  • Method

    PC12 cells (2×105cells/ml) are stained with Hoechst33342 after incubation for 12h with or without 75 μM 6-OHDA and observed under fluorescence microscopy. Cells are treated with various concentrations of 6-OHDA for indicated times and stained with Hoechst33342. Total cells (500-1000 cells) and chromatin condensed cells are counted in the same field, and percentage of chromatin condensation is calculated.
Animal Study:

[2]
  • Animal Models

    female Sprague-Dawley rats

  • Dosages

    12 mM

  • Administration

    i.s.

Selleck's 6-OHDA (Oxidopamine hydrobromide) has been cited by 1 publication

Effect of TO901317 on GF to promote the differentiation of human bone marrow mesenchymal stem cells into dopamine neurons on Parkinson's disease [ Ther Adv Chronic Dis, 2021, 12:2040622321998139] PubMed: 33796244

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.