NSC59984

Catalog No.S8106 Batch:S810601

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Technical Data

Formula

C12H15N3O4
Molecular Weight 265.27 CAS No. 803647-40-7
Solubility (25°C)* In vitro DMSO 53 mg/mL (199.79 mM)
Ethanol 53 mg/mL (199.79 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description NSC59984 is a p53 pathway activator via induction of mutant p53 protein degradation and p73 activation.
Targets
p53 [1]
In vitro NSC59984 specifically restores p53 pathway signaling and induces p73-dependent cell death in mutant p53-expressing human colorectal cancer cells. NSC59984 also induces cell death in a panel of cancer cell lines bearing different p53 mutations with little or no genotoxicity in normal cells. [1]
In vivo In nude mice bearing DLD-1 xenograft tumors, NSC59984 (45 mg/kg, i.p.) significantly represses tumor growth in a p73-dependent manner. [1]

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    SW480, DLD-1, HCT116, p53-null HCT116, MRC5, Hop92, RXF393 and Wi38 cells

  • Concentrations

    ~100 μM

  • Incubation Time

    72 h

  • Method

    Cells are seeded at 5,000 cells per well on 96-well plates. Cells are mixed with an equal volume of CellTiter-Glo reagents, following the manufacturer's protocol, and bioluminescence imaging is measured using the IVIS imager.

Selleck's NSC59984 has been cited by 4 publications

DRP1 Inhibition Enhances Venetoclax-Induced Mitochondrial Apoptosis in TP53-Mutated Acute Myeloid Leukemia Cells through BAX/BAK Activation [ Cancers (Basel), 2023, 15(3)745] PubMed: 36765703
Autophagy Inhibition in Trophoblasts Induces Aberrant Shift in CXCR4+ Decidual NK Cell Phenotype Leading to Pregnancy Loss [ J Clin Med, 2023, 12(23)7491] PubMed: 38068544
Multi-Cell Type Glioblastoma Tumor Spheroids for Evaluating Sub-Population-Specific Drug Response [ Front Bioeng Biotechnol, 2020, 8:538663] PubMed: 33042963
Immersion Bioprinting of Tumor Organoids in Multi-Well Plates for Increasing Chemotherapy Screening Throughput. [ Micromachines (Basel), 2020, 11(2)] PubMed: 32085455

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.