Nile Red

Catalog No.S6818 Batch:S681801

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Technical Data

Formula

C20H18N2O2
Molecular Weight 318.37 CAS No. 7385-67-3
Solubility (25°C)* In vitro DMSO 40 mg/mL (125.63 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Nile Red (Nile Blue A oxazone, Phenoxazone 9) is an excellent vital fluorescent stain for the detection of intracellular lipid droplets in the presence of a hydrophobic environment. Nile Red is applied for staining intracellular lipids, hydrophobic domains of proteins and lysosomal phospholipid inclusions.
Targets
lipid droplet [1]
In vitro

1. Preparation of Phalloidin-TRITC working solution
1.1Preparation of the stock solution
Dissolve Phalloidin-TRITC in Methanol to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Phalloidin-TRITC working solution
Dilute the stock solution in serum-free cell culture medium to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of Phalloidin-TRITC working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
b.Add 1 mL of working solution, and then incubate at room temperature for 30-60 minutes.
c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.

Protocol (from reference)

Selleck's Nile Red has been cited by 2 publications

CD24 negativity reprograms mitochondrial metabolism to PPARα and NF-κB-driven fatty acid β-oxidation in triple-negative breast cancer [ Cancer Lett, 2024, 587:216724] PubMed: 38373689
Photothermally sensitive gold nanocage augments the antitumor efficiency of immune checkpoint blockade in immune "cold" tumors [ Front Immunol, 2023, 10.3389/fimmu.2023.1279221] PubMed: 37942337

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.