Nigericin sodium salt

Catalog No.S6653 Batch:S665303

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Technical Data

Formula

C40H67NaO11
Molecular Weight 746.94 CAS No. 28643-80-3
Solubility (25°C)* In vitro Ethanol 100 mg/mL (133.87 mM)
DMSO Insoluble
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Nigericin sodium salt is an antibiotic derived from Streptomyces hygroscopicus that works by acting as an H+, K+, and Pb2+ ionophore.Nigericin can activate NLRP3 inflammasome to induce pro-inflammatory and immunostimulatory processes.
Targets
Potassium channel [1] Hydrogen channel [1] Pb 2+ [1] NLRP3 [2]
In vitro

In vitro model of tumor heterogeneity the antitumor effects of Nigericin (NIG) that works by acting as an H+, K+, and Pb2+ ionophore. NIG decreased the viability and clonogenicity of H460 cells, as well as their ability to migrate and invade[1] .

Using a combination of live-cell imaging and biochemical approaches, we demonstrate that prototypical NLRP3 stimuli, the potassium ionophore nigericin, or millimolar concentrations of ATP exert both NLRP3-independent and -dependent effects on macrophages that result in cytokine release and cell death. [2]
In vivo

Nigericin is a NOD-like receptor protein 3 (NLRP3) activator.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    H460 cells

  • Concentrations

    1 µM

  • Incubation Time

    24 h

  • Method

    H460 cells are seeded in 8-µm pore size Transwell® Inserts in RPMI 1640 (without FBS) with or without drugs (DMSO as control) or NIG (1µM). Inserts are placed in 24-well plates with RPMI 1640 containing 10% FBS in the presence of DMSO (control) or NIG (1 µM).
Animal Study:

[3]
  • Animal Models

    Male C57BL/6 mice

  • Dosages

    4 mg/kg

  • Administration

    i.p.

Selleck's Nigericin sodium salt has been cited by 26 publications

Chinese medicinal formula Fu Xin decoction against chronic heart failure by inhibiting the NLRP3/caspase-1/GSDMD pyroptotic pathway [ Biomed Pharmacother, 2024, 174:116548] PubMed: 38599064
3'-Deoxyadenosin alleviates methamphetamine-induced aberrant synaptic plasticity and seeking behavior by inhibiting the NLRP3 inflammasome [ Neural Regen Res, 2024, 19(10):2270-2280] PubMed: 38488561
ER stress induces caspase-2-tBID-GSDME-dependent cell death in neurons lytically infected with herpes simplex virus type 2 [ EMBO J, 2023, e113118.] PubMed: 37646198
Lipocalin-2-mediated astrocyte pyroptosis promotes neuroinflammatory injury via NLRP3 inflammasome activation in cerebral ischemia/reperfusion injury [ J Neuroinflammation, 2023, 20(1):148] PubMed: 37353794
Stellate ganglion block ameliorated central post-stroke pain with comorbid anxiety and depression through inhibiting HIF-1α/NLRP3 signaling following thalamic hemorrhagic stroke [ J Neuroinflammation, 2023, 20(1):82] PubMed: 36944982
A novel sorbicillinoid compound as a potent anti-inflammation agent through inducing NLRP3 protein degradation [ Br J Pharmacol, 2023, 10.1111/bph.16058] PubMed: 36788033
Takinib inhibits microglial M1 polarization and oxidative damage after subarachnoid hemorrhage by targeting TAK1-dependent NLRP3 inflammasome signaling pathway [ Front Immunol, 2023, 14:1266315] PubMed: 38035075
Eleutheroside E from pre-treatment of Acanthopanax senticosus (Rupr.etMaxim.) Harms ameliorates high-altitude-induced heart injury by regulating NLRP3 inflammasome-mediated pyroptosis via NLRP3/caspase-1 pathway [ Int Immunopharmacol, 2023, 121:110423] PubMed: 37331291
Sipeimine attenuates PM2.5-induced lung toxicity via suppression of NLRP3 inflammasome-mediated pyroptosis through activation of the PI3K/AKT pathway [ Chem Biol Interact, 2023, 376:110448] PubMed: 36898572
Electroacupuncture improves gout arthritis pain via attenuating ROS-mediated NLRP3 inflammasome overactivation [ Chin Med, 2023, 18(1):86] PubMed: 37464384

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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