MSAB

Catalog No.S6901 Batch:S690101

Print

Technical Data

Formula

C15H15NO4S
Molecular Weight 305.35 CAS No. 173436-66-3
Solubility (25°C)* In vitro DMSO 61 mg/mL (199.77 mM)
Ethanol 10 mg/mL (32.74 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description MSAB is a selective inhibitor of Wnt/β-catenin signaling that binds to β-catenin, promoting its degradation, and specifically downregulates Wnt/β-catenin target genes. MSAB shows potent anti-tumor effects.
Targets
β-catenin [1]
In vitro

MSAB impedes RAW264.7 cells and human PMBCs differentiating into OCs. MSAB decreases the expression of β‐catenin in RAW264.7 and ARP1 WT cells.[2]
In vivo

MSAB (10-20 mg/kg; i.p. daily for 2 weeks) inhibits tumor growth of Wnt-dependent cancer cells in xenograft mice and MMTV-Wnt1 transgenic mice.[3]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Human blood mononuclear cells (PBMCs), Human MM cell lines, HEK293, RAW264.7 cells

  • Concentrations

    0, 2.5, 5, 10, 20, 40 μM

  • Incubation Time

    6 days, 15 days

  • Method

    RAW264 cells are seeded in a 24‐well plate at the density of 3,000 cells/well. Humen PBMCs cells are seeded in a 24‐well plate at the density of 1 × 106 cells/well. After 6 days (RAW264 cells) or 15 days (PBMCs), the degree of osteoclast differentiation is determined by detecting the TRAP activity of the cells.

Selleck's MSAB has been cited by 7 publications

Colorectal cancer cells secreting DKK4 transform fibroblasts to promote tumour metastasis [ Oncogene, 2024, 10.1038/s41388-024-03008-1] PubMed: 38519641
Hyperoxia exposure upregulates Dvl-1 and activates Wnt/β-catenin signaling pathway in newborn rat lung [ BMC Mol Cell Biol, 2023, 24(1):4] PubMed: 36726071
Inhibition of beta-catenin shows therapeutic potential in head and neck squamous cell carcinoma in vitro [ Eur Arch Otorhinolaryngol, 2023, 280(1):399-408] PubMed: 36001136
Splicing factor arginine/serine-rich 8 promotes multiple myeloma malignancy and bone lesion through alternative splicing of CACYBP and exosome-based cellular communication [ Clin Transl Med, 2022, 12(2):e684] PubMed: 35184390
Empagliflozin activates Wnt/β-catenin to stimulate FUNDC1-dependent mitochondrial quality surveillance against type-3 cardiorenal syndrome [ Mol Metab, 2022, 64:101553] PubMed: 35863636
G-protein-coupled receptor 124 promotes osteogenic differentiation of BMSCs through the Wnt/β-catenin pathway [ In Vitro Cell Dev Biol Anim, 2022, 10.1007/s11626-022-00684-9] PubMed: 35916978
Cartilage Endplate Stem Cells Transdifferentiate Into Nucleus Pulposus Cells via Autocrine Exosomes [ Front Cell Dev Biol, 2021, 9:648201] PubMed: 33748142

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.