Melittin

Catalog No.S9794 Batch:S979401

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Technical Data

Formula

C131H229N39O31

Molecular Weight 2846.46 CAS No. 20449-79-0, 37231-28-0
Solubility (25°C)* In vitro Water 100 mg/mL (35.13 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Melittin (MLT, Forapin, Forapine) is an activator of phospholipase A2 (PLA2) that stimulates the activity of the low molecular weight PLA2, while it does not the increase the activity of the high molecular weight enzyme.
Targets
PLA2 [1]
In vitro

In vitro studies using TNF-α/IFN-γ-stimulated human keratinocytes shows that melittin inhibits the increased expression of chemokines, such as CCL17 and CCL22, and pro-inflammatory cytokines, including IL-1β, IL-6 and IFN-γ, through the blockade of the NF‐κB and STAT signalling pathways.[2]

In vivo

Melittin exhibits potent anti-atopic activities, shown by decreased Atopic dermatitis (AD)-like skin lesions, induced by DNCB in mice.[2]

Protocol (from reference)

Cell Assay:

[2]

  • Cell lines

    HaCaT cell line

  • Concentrations

    0.1 μg/mL, 0.5 μg/mL, 1 μg/mL

  • Incubation Time

    30 min

  • Method

    The human keratinocyte HaCaT cell line is cultured in high glucose DMEM supplemented with 10% (v/v) FBS and antibiotics at 37°C in a humidified 5% CO2 incubator. HaCaT (5.0 × 105 cells/mL) cells are seeded in the complete medium. 24 hours later, the medium is changed to serumfree mediumwith bee venom (1, 10 and 100 ng/mL) and melittin (0.1, 0.5 and 1 μg/mL). After 30 min, the cells are stimulated with 10 ng/mL TNF-α/IFN-γ for 9 h.

Animal Study:

[2]

  • Animal Models

    6-week-old female Balb/c mice

  • Dosages

    100 μg, 200 μg, 500 μg

  • Administration

    Topical

Selleck's Melittin has been cited by 2 publications

Melittin induces autophagy to alleviate chronic renal failure in 5/6-nephrectomized rats and angiotensin II-induced damage in podocytes [ Nutr Res Pract, 2024, 18(2):210-222] PubMed: 38584815
PGE2 -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase [ EMBO J, 2022, e110439] PubMed: 35781818

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Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.