Lysionotin

Catalog No.S3877 Batch:S387701

Technical Data

Formula	C₁₈H₁₆O₇
Molecular Weight	344.32	CAS No.	152743-19-6
Solubility (25°C)*	In vitro	DMSO	68 mg/mL (197.49 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Lysionotin is a natural flavonoid predominantly found in fewflower lysionotus herbs and possesses many pharmacological properties, such as antibacterial, anti-inflammatory, antihypertensive, and free radical scavenging activities.
In vitro	Lysionotin is an efficient inhibitor of α-toxin expression and shows significant protection against S. aureus in vitro and in vivo^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines A549 human lung epithelial cells Concentrations 0-16 μg/ml Incubation Time 5 h Method A549 human lung epithelial cells (ATCC CCL 185) are maintained in DMEM supplemented with 10% fetal serum at 37 ℃ and seeded in 96-well plates at a density of approximately 2 × 10⁴ cells per well. Then, the cells are cultured with 200 μl of bacteria with various concentrations of lysionotin and incubated for 5 h at 37 ℃. For LDH assays, the supernatants from wells are assessed for LDH release with the Cytotoxicity Detection Kit.
Animal Study:^{^[1]}	Animal Models 6- to 8-week-old female C57BL/6J mice Dosages 100 mg/kg Administration by hypodermic injection in the back skin

Cell Assay:^{^[1]}

Cell lines
A549 human lung epithelial cells
Concentrations
0-16 μg/ml
Incubation Time
5 h
Method
A549 human lung epithelial cells (ATCC CCL 185) are maintained in DMEM supplemented with 10% fetal serum at 37 ℃ and seeded in 96-well plates at a density of approximately 2 × 10⁴ cells per well. Then, the cells are cultured with 200 μl of bacteria with various concentrations of lysionotin and incubated for 5 h at 37 ℃. For LDH assays, the supernatants from wells are assessed for LDH release with the Cytotoxicity Detection Kit.

Animal Study:^{^[1]}

Animal Models
6- to 8-week-old female C57BL/6J mice
Dosages
100 mg/kg
Administration
by hypodermic injection in the back skin

References

[1] Teng Z, et al. Appl Microbiol Biotechnol. 2017, 101(17):6697-6703.

Selleck's Lysionotin has been cited by 1 publication

Altered T Follicular Helper Cell Subsets and Function in Chronic Lymphocytic Leukemia [ Front Oncol, 2021, 11:674492]	PubMed: 33996605

Altered T Follicular Helper Cell Subsets and Function in Chronic Lymphocytic Leukemia [ Front Oncol, 2021, 11:674492]

PubMed: 33996605

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.