Loxistatin Acid (E-64C)

Catalog No.S7392 Batch:S739201

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Technical Data

Formula

C15H26 N2 O5
Molecular Weight 314.38 CAS No. 76684-89-4
Solubility (25°C)* In vitro DMSO 62 mg/mL (197.21 mM)
Ethanol 62 mg/mL (197.21 mM)
Water 2 mg/mL (6.36 mM)
In vivo (Add solvents to the product individually and in order)
Clear solution
saline
2.0mg/ml Taking the 1 mL working solution as an example, add 2 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Loxistatin Acid (E-64C, NSC 694279, EP 475), a derivative of E-64, is an irreversible and membrane-permeant cysteine protease inhibitor. The cysteine protease cathepsin L is required for SARS-CoV-2 viral entry.
Targets
cysteine protease [1]
In vitro Loxistatin Acid (E-64C), as a thiol protease inhibitor, inhibits calpain activity in intact platelets. [1]E-64C inhibits β-secretase activity in regulated secretory vesicles of neuronal chromaffin cells, and decrease Aβ production, indicating a therapeutic strategy for Alzheimer's disease. [4]
In vivo In pectoral muscle of dystrophic chickens, Loxistatin Acid (E-64C) normalizes the increased activities of cathepsin B and cathepsin H. [2] E-64C inhibits ischemic degradation of cerebral proteins following occlusion of the middle cerebral artery in rats. [3]

Protocol (from reference)

Animal Study:[3]
  • Animal Models

    Male Sprague-Dawley rats.

  • Dosages

    ~400 mg/kg

  • Administration

    i.p.

Selleck's Loxistatin Acid (E-64C) has been cited by 4 publications

Selective Neutral pH Inhibitor of Cathepsin B Designed Based on Cleavage Preferences at Cytosolic and Lysosomal pH Conditions [ ACS Chem Biol, 2021, 16(9):1628-1643] PubMed: 34416110
Abl and Arg mediate cysteine cathepsin secretion to facilitate melanoma invasion and metastasis [ Sci Signal, 2018, 11(518)eaao0422] PubMed: 29463776
Abl and Arg mediate cysteine cathepsin secretion to facilitate melanoma invasion and metastasis [ Sci Signal, 2018, 11(518)eaao0422] PubMed: 29463776
An epithelial-to-mesenchymal transition-inducing potential of granulocyte macrophage colony-stimulating factor in colon cancer. [ Sci Rep, 2017, 7(1):8265] PubMed: 28811578

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.