Licochalcone A

Catalog No.S7828 Batch:S782801

Technical Data

Formula	C₂₁H₂₂O₄
Molecular Weight	338.4	CAS No.	58749-22-7
Solubility (25°C)*	In vitro	DMSO	67 mg/mL (197.99 mM)
		Ethanol	67 mg/mL (197.99 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Licochalcone A is an estrogenic flavanoid extracted from licorice root, showing antimalarial, anticancer, antibacterial and antiviral activities. Phase 3.
In vitro	Licochalcone A markedly inhibits the in vitro growth of L. major amastigotes in human MDMs and U937 cells. ^[1] Licochalcone A shows antibacterial effects against all gram-positive bacteria tested and especially against all Bacillus spp. Tested with MICs of 2 to 3 micrograms/mL. ^[3] In CT-26 colon cancer cells, Licochalcone A reduces the cell viability and DNA synthesis. ^[4] Licochalcone A also interferes with MAPK signaling cascades, initiates ROS generation, induces oxidative stress and consequently causes BGC cell apoptosis. ^[5]
In vivo	In mice infected with L. major, licochalcone A (5 mg/kg, i.p.) completely prevents lesion development. In mice infected with L. donovani, licochalcone A (150 mg/kg, p.o.) results in > 65 and 85% reductions of parasite loads in the liver and the spleen, respectively. ^[2] In CT-26 cell-inoculated Balb/c mice, licochalcone A (1 mg/kg, p.o.) inhibits the tumor growth, and alleviates cisplatin-induced nephrotoxicity and hepatotoxicity. ^[4]

Protocol (from reference)

Cell Assay:^{^[4]}	Cell lines CT-26 cells Concentrations ~20 μM Incubation Time 72 hours Method The viability of CT-26 mouse colon cancer cells is determined via a MTT assay. In brief, colon cancer cells are seeded onto each well of a 96-well plate with DMEM containing 10% FBS and cultured to adhere overnight. The cells are then treated with various concentrations of LCA in serum-free medium for 24 and 72 hr, respectively. Twenty microlitres of a MTT solution (5 mg/ml) is added to each well, and the cells are incubated for 4 hr at 37°. The medium is then removed, and 200 µL of dimethyl sulfoxide is added to each well. The absorbance is determined at 570 nm using a microplate reader.
Animal Study:^{^[2]}	Animal Models Mice infected with L. major Dosages 5 mg/kg/day Administration i.p. or p.o.

Cell Assay:^{^[4]}

Cell lines
CT-26 cells
Concentrations
~20 μM
Incubation Time
72 hours
Method
The viability of CT-26 mouse colon cancer cells is determined via a MTT assay. In brief, colon cancer cells are seeded onto each well of a 96-well plate with DMEM containing 10% FBS and cultured to adhere overnight. The cells are then treated with various concentrations of LCA in serum-free medium for 24 and 72 hr, respectively. Twenty microlitres of a MTT solution (5 mg/ml) is added to each well, and the cells are incubated for 4 hr at 37°. The medium is then removed, and 200 µL of dimethyl sulfoxide is added to each well. The absorbance is determined at 570 nm using a microplate reader.

Animal Study:^{^[2]}

Animal Models
Mice infected with L. major
Dosages
5 mg/kg/day
Administration
i.p. or p.o.

References

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Selleck's Licochalcone A has been cited by 5 publications

Licochalcone A protects against LPS-induced inflammation and acute lung injury by directly binding with myeloid differentiation factor 2 (MD2) [ Br J Pharmacol, 2023, 180(8):1114-1131]	PubMed: 36480410
Licochalcone a improves cardiac functions after ischemia-reperfusion via reduction of ferroptosis in rats [ Eur J Pharmacol, 2023, 957:176031]	PubMed: 37660967
JNK inhibitor IX restrains pancreatic cancer through p53 and p21 [ Front Oncol, 2022, 12:1006131]	PubMed: 36568248
Licochalcone A Promotes the Ubiquitination of c-Met to Abrogate Gefitinib Resistance [ Biomed Res Int, 2022, 2022:5687832]	PubMed: 35309168
Licochalcone A inhibits cell growth through the downregulation of the Hippo pathway via PES1 in cholangiocarcinoma cells [ Environ Toxicol, 2021, 10.1002/tox.23422]	PubMed: 34845814

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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