Latanoprost

Catalog No.S4709 Batch:S470901

Technical Data

Formula	C₂₆H₄₀O₅
Molecular Weight	432.59	CAS No.	130209-82-4
Solubility (25°C)*	In vitro


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description

Latanoprost (Xalatan, PhXA41) is a prostaglandin F2alpha analogue and a prostanoid selective FP receptor agonist with an ocular hypertensive effect. Latanoprost increases uveoscleral outflow and thereby reduces intraocular pressure.

Targets

retinoid X receptor α ^[2]

PGF-prostanoid receptor ^[3]

In vitro

Latanoprost functioned as both an indirect activator of AMP-activated protein kinase and a selective retinoid X receptor α (RXRα) antagonist able to selectively antagonise the transcription of a RXRα/peroxisome proliferator-activated receptor γ heterodimer^[2]. Latanoprost induced morphological abnormality and viability decline of HCS cells in vitro. It induces cell cycle arrest of HCS cells. Latanoprost induces abnormal changes of plasma membrane, DNA fragmentation and ultrastructural abnormality of HCS cells. Caspase activation in HCS cells is also activated by Latanoprost treatment. Latanoprost induces MTP disruption and quantitative changes of mitochondrion-associated pro-apoptotic regulators in HCS cells^[1]. Latanoprost is effective in inhibiting adipogenesis, reducing lipogenesis, promoting fatty acid oxidation and enhancing GLUT4 translocation and glucose uptake both in adipocytes and myotubes^[2].

In vivo

Latanoprost, a clinical drug for treating primary open-angle glaucoma and intraocular hypertension, effectively ameliorates glucose and lipid disorders in two mouse models of type 2 diabetes. Its treatment improves glucose tolerance. Chronic administration of latanoprost decreases serum lipids and enhances insulin signalling in white adipose tissue and skeletal muscle. It effectually activates AMPK and regulates glucose and lipid metabolism-relevant genes in diabetic mice^[2].

Density

1.093 g/mL

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Human corneal stromal (HCS) cells Concentrations from 50 mg/l to 0.78125 mg/l Incubation Time 4, 8, 12 h Method HCS cells were inoculated into a 24-well culture plate (Nunc) and cultured in 10% FBS-DMEM/F12 medium at 37°C in a humidified 5% CO2 incubator. After the cells grew into logarithmic phase, the culture medium of each culture plate well was replaced entirely with the medium containing latanoprost at concentrations varying from 50 mg/l to 0.78125 mg/l, respectively. The cells were cultured under the same condition as described earlier, and their morphology and growth status were monitored every 4 h under an Eclipse TS100 inverted microscope.
Animal Study:^{^[2]}	Animal Models db/db or ob/ob mice Dosages 10 or 40 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
Human corneal stromal (HCS) cells
Concentrations
from 50 mg/l to 0.78125 mg/l
Incubation Time
4, 8, 12 h
Method
HCS cells were inoculated into a 24-well culture plate (Nunc) and cultured in 10% FBS-DMEM/F12 medium at 37°C in a humidified 5% CO2 incubator. After the cells grew into logarithmic phase, the culture medium of each culture plate well was replaced entirely with the medium containing latanoprost at concentrations varying from 50 mg/l to 0.78125 mg/l, respectively. The cells were cultured under the same condition as described earlier, and their morphology and growth status were monitored every 4 h under an Eclipse TS100 inverted microscope.

Animal Study:^{^[2]}

Animal Models
db/db or ob/ob mice
Dosages
10 or 40 mg/kg
Administration
i.p.

References

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