Kaempferide

Catalog No.S3879 Batch:S387901

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Technical Data

Formula

C16H12O6
Molecular Weight 300.26 CAS No. 491-54-3
Solubility (25°C)* In vitro DMSO 60 mg/mL (199.82 mM)
Ethanol 13 mg/mL (43.29 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Kaempferide (4'-Methylkaempferol, 4'-O-Methylkaempferol, Kaempferol 4'-methyl ether), a natural compound derived from the roots of kaempferia galanga, has a variety of effects including anti-carcinogenic, anti-inflammatory, anti-oxidant, anti-bacterial and anti-viral properties.
In vitro Kaempferide is able to induce apoptosis in the "side population" (SP, a subpopulation enriched with CSC in various cancers) cells in myeloma. It reverses the activity of drug efflux transporter[2]. Kaempferide is non-toxic to normal fibroblasts while inducing morphological changes, membrane flip-flop and nuclear membrane damage, characteristic of apoptosis in HeLa cells. It is highly cytotoxic to cervical cancer cells. Kaempferide-induced cytotoxicity is independent of cell cycle arrest[3].
In vivo Kaempferide (Kae) remarkably improves cardiac function, alleviates myocardial injury via a decrease in myocardial enzyme levels, and attenuates myocardial infarct size in a dose-dependent manner. It attenuates I/R-induced myocardial injury through inhibition of the Nrf2 and cleaved caspase-3 signaling pathways via a PI3K/Akt/GSK 3β-dependent mechanism. Preconditioning treatment with Kae significantly decreases serum TNF-α, IL-6, C-reactive protein (CRP), MDA, and ROS levels, while increases serum levels of SOD. Nuclear factor erythroid 2-related factor 2 (Nrf2) and cleaved caspase-3 expression levels are downregulated, while phospho-Akt (p-Akt) and phospho-glycogen synthase kinase-3β (p-GSK-3β) expression levels are upregulated[1]. Kaempferide is non-toxic as assessed by acute and chronic toxicity studies in Swiss albino mice in vivo[3].

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    KMS-11 cells

  • Concentrations

    4-64 μM

  • Incubation Time

    24 h

  • Method

    Unfractionated cells are seeded at 20000 cells per well in a 96 well plate. After 24h of incubation with various concentration of KFD, PAR, and DMSO (vehicle control), MTS solution is added and absorbance at 490 nm is determined.
Animal Study:

[1]
  • Animal Models

    Ischemia/Reperfusion (I/R) Rat Model (Adult Sprague Dawley (SD) rats)

  • Dosages

    0.1 mg/kg, 0.3 mg/kg, 1 mg/kg

  • Administration

    i.p.

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.