JW55

Catalog No.S6745 Batch:S674501

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Technical Data

Formula

C25H26N2O5

Molecular Weight 434.49 CAS No. 664993-53-7
Solubility (25°C)* In vitro DMSO 87 mg/mL (200.23 mM)
Ethanol 4 mg/mL (9.2 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description JW55 is a potent and selective inhibitor of the canonical Wnt pathway that functions via inhibition of the PARP domain of tankyrase 1 and tankyrase 2 (TNKS1/2).
Targets
Wnt [1]
In vitro

JW55 inhibits the PARP domain of TNKS1/2, leading to the stabilization of AXIN2 followed by increased degradation of β-catenin. Wnt3a-induced HEK293 cells containing a transiently transfected ST-Luc (SuperTop-luciferase) reporter show inhibition by JW55 with an IC50 value of 470 nmol/L. JW55 decreases auto-PARsylation of TNKS1/2 in vitro with IC50 values of 1.9 μmol/L and 830 nmol/L, respectively. JW55-mediated inhibition of canonical Wnt signaling results in reduced cell-cycle progression, proliferation, and colony formation in the CRC cell line SW480 in vitro[1].

In vivo

JW55 reduces XWnt8-induced axis duplication in Xenopus embryos and tamoxifen-induced polyposis formation in conditional APC mutant mice[1].

Protocol (from reference)

Cell Assay:

[1]

  • Cell lines

    SW480 cells

  • Concentrations

    10, 5, or 1 μmol/L

  • Incubation Time

    9 days

  • Method

    --

Animal Study:

[1]

  • Animal Models

    Apc(CKO/CKO)Lgr5-CreERT2+ mice

  • Dosages

    100 mg/kg

  • Administration

    oral

Selleck's JW55 has been cited by 3 publications

Integration of 3D bioprinting and multi-algorithm machine learning identified glioma susceptibilities and microenvironment characteristics [ Cell Discov, 2024, 10(1):39] PubMed: 38594259
Effect of cell microenvironment on the drug sensitivity of hepatocellular cancer cells [ Oncotarget, 2021, 12(7):674-685] PubMed: 33868588
Babao Dan () Alleviates 5-Fluorouracil-Induced Intestinal Damage via Wnt/β-Catenin Pathway [ Chin J Integr Med, 2021, 10.1007/s11655-021-3282-0] PubMed: 33420580

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.