Jatrorrhizine

Catalog No.S3884 Batch:S388401

Technical Data

Formula	C₂₀H₂₀NO₄
Molecular Weight	338.38	CAS No.	3621-38-3
Solubility (25°C)*	In vitro	DMSO	67 mg/mL (198.0 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Jatrorrhizine (Neprotin, Yatrorizine), one of the active constituents of Coptis chinensis Franch, has multiple bioactivities, such as hypoglycemic, antimicrobial, and antioxidant activities. It is an inhibitor of AChE with IC50 of 872 nM and demonstrates >115-fold selectivity for AChE over BuChE.

Targets

AChE ^[1]
(Cell-free assay)

872 nM

In vitro

Jatrorrhizine hydrochloride suppresses C8161 cell proliferation in a dose-dependent manner, with a half-maximal inhibitory concentration of 47.4±1.6 μmol/l. however, it does not induce significant cellular apoptosis at doses up to 320 μmol/l. Mechanistic studies show that JH-induced C8161 cell cycle arrest at the G0/G1 transition, which is accompanied by overexpression of the cell cycle-suppressive genes p21 and p27 at higher doses^[2].

In vivo

JH reduces metastatic melanoma cell-mediated neovascularization^[2]. JH ameliorates hyperlipidemia via the suppression of lipogenesis and the enhancement of lipid oxidation in the liver^[3].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Human melanoma C8161 cells Concentrations 0, 80, 160, 320 μmol/L Incubation Time 48 h Method C8161 cells are grown in T75 flasks and treated with the indicated JH for 48 h. After the treatment, the cells are fixed with 70% ethanol overnight at 41℃, washed with PBS, and resuspended in 500 ml PBS with 100 mg/ml RNase. After incubation for 30 min at 37℃, 2.5 ml (10 mg/ml) PI solution is added and incubated on ice for another 30 min in the dark. The DNA contents of PI-stained cells are analyzed using flow cytometry.
Animal Study:^{^[3]}	Animal Models 7-week-old male C57BL/6 mice Dosages 20 and 100 mg/kg Administration intragastric injections

Cell Assay:^{^[2]}

Cell lines
Human melanoma C8161 cells
Concentrations
0, 80, 160, 320 μmol/L
Incubation Time
48 h
Method
C8161 cells are grown in T75 flasks and treated with the indicated JH for 48 h. After the treatment, the cells are fixed with 70% ethanol overnight at 41℃, washed with PBS, and resuspended in 500 ml PBS with 100 mg/ml RNase. After incubation for 30 min at 37℃, 2.5 ml (10 mg/ml) PI solution is added and incubated on ice for another 30 min in the dark. The DNA contents of PI-stained cells are analyzed using flow cytometry.

Animal Study:^{^[3]}

Animal Models
7-week-old male C57BL/6 mice
Dosages
20 and 100 mg/kg
Administration
intragastric injections

References

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SHIPPING AND STORAGE
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