IWR-1-endo

Catalog No.S7086 Batch:S708603

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Technical Data

Formula

C25H19N3O3
Molecular Weight 409.44 CAS No. 1127442-82-3
Solubility (25°C)* In vitro DMSO 15 mg/mL (36.63 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description IWR-1-endo (endo-IWR 1, IWR-1) is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.
Targets
Wnt [1]
(L-cells expressing Wnt3A)
180 nM
In vitro

Although both IWR-1 and XAV939 act as reversible Wnt pathway inhibitors and exhibit similar pharmacological effects both in vitro and in vivo, IWR-1 exerts its effect via interaction with Axin, while XAV939 binds TNKS directly. [1]
In vivo

IWR-1 is a β‐catenin inhibitor.[3]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Pig EpiSCs

  • Concentrations

    2.5 μM

  • Incubation Time

    2 days

  • Method

    For neural induction, the 3i/LAF culture medium was replaced with neural induction medium I (2.5 μM IWR-1-endo, 5 μM SB431542, and 10 ng/mL FGF2 in BM) on day 2 after pgEpiSC passage.

Customer Product Validation

Data from [Data independently produced by , , Diabetes, 2018, 67(4):554-568]

Data from [Data independently produced by , , Biochim Biophys Acta Mol Cell Biol Lipids, 2018, 1863(8):834-843]

Data from [Data independently produced by , , Am J Cancer Res, 2015, 5(10):3123-3134.]

Selleck's IWR-1-endo has been cited by 127 publications

Derivation of human primordial germ cell-like cells in an embryonic-like culture [ Nat Commun, 2024, 15(1):167] PubMed: 38167821
Autophagy is essential for human myelopoiesis [ Stem Cell Reports, 2024, 19(2):196-210] PubMed: 38215759
Inhibitor of Wnt receptor 1 suppresses the effects of Wnt1, Wnt3a and β‑catenin on the proliferation and migration of C6 GSCs induced by low‑dose radiation [ Oncol Rep, 2024, 51(2)22] PubMed: 38099414
CXXC finger protein 1 -CFP1) bridges the reshaping of genomic H3K4me3 signature to the advancement of lung adenocarcinoma [ Signal Transduct Target Ther, 2023, 8(1):369] PubMed: 37735441
CXXC finger protein 1 (CFP1) bridges the reshaping of genomic H3K4me3 signature to the advancement of lung adenocarcinoma [ Signal Transduct Target Ther, 2023, 8(1):369] PubMed: 37735441
A live-cell image-based machine learning strategy for reducing variability in PSC differentiation systems [ Cell Discov, 2023, 9(1):53] PubMed: 37280224
Direct 3D-Bioprinting of hiPSC-Derived Cardiomyocytes to Generate Functional Cardiac Tissues [ Adv Mater, 2023, 35(52):e2305911] PubMed: 37655652
Long-term in vivo chimeric cells tracking in non-human primate [ Protein Cell, 2023, pwad049] PubMed: 37758041
Inhibition of Wnt activity improves peri-implantation development of somatic cell nuclear transfer embryos [ Natl Sci Rev, 2023, 10(9):nwad173] PubMed: 37593113
Inhibition of Wnt activity improves peri-implantation development of somatic cell nuclear transfer embryos [ Natl Sci Rev, 2023, 10(9):nwad173] PubMed: 37593113

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.