ITSA-1 (ITSA1)

Catalog No.S8323 Batch:S832301

Technical Data

Formula

C₁₃H₇Cl₂N₃O

Molecular Weight

292.12

CAS No.

200626-61-5

Solubility (25°C)*

In vitro

DMSO

58 mg/mL (198.54 mM)

Ethanol

5 mg/mL (17.11 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution

5%DMSO 1 Corn oil

3.0mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 60 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

ITSA-1 (ITSA1) is an HDAC activator via TSA suppression, but shows no activity towards other HDAC inhibitors.

Targets

HDAC ^[1]
(Cell-free assay)

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines A549 cells Concentrations 50 μM Incubation Time 17.5 h Method A549 cells ( 75% confluent) in 10-cm dishes are treated with DMSO (0.1%), TSA (500 nM), or tubacin (2 μM) for 4.5 h, and then either DMSO (0.1%) or ITSA1 (50 μM) is added for an additional 17.5 h. Samples are rinsed in PBS, trypsinized, and fixed for 2 h in 70% ethanol 30% PBS (4°C). Cells then are washed once in PBS and stored overnight in PBS (4°C).

Cell Assay:^{^[1]}

Cell lines
A549 cells
Concentrations
50 μM
Incubation Time
17.5 h
Method
A549 cells ( 75% confluent) in 10-cm dishes are treated with DMSO (0.1%), TSA (500 nM), or tubacin (2 μM) for 4.5 h, and then either DMSO (0.1%) or ITSA1 (50 μM) is added for an additional 17.5 h. Samples are rinsed in PBS, trypsinized, and fixed for 2 h in 70% ethanol 30% PBS (4°C). Cells then are washed once in PBS and stored overnight in PBS (4°C).

References

[1] Haggarty SJ, et al. Proc Natl Acad Sci U S A. 2003, 100(8):4389-94.

Selleck's ITSA-1 (ITSA1) has been cited by 3 publications

Tumour cells are sensitised to ferroptosis via RB1CC1-mediated transcriptional reprogramming [ Clin Transl Med, 2022, 12(2):e747]	PubMed: 35220675
Trichostatin A ameliorates Alzheimer's disease-related pathology and cognitive deficits by increasing albumin expression and Aβ clearance in APP/PS1 mice [ Alzheimers Res Ther, 2021, 13(1):7]	PubMed: 33397436
Epigenetic Reprogramming with Antisense Oligonucleotides Enhances the Effectiveness of Androgen Receptor Inhibition in Castration-Resistant Prostate Cancer [ Cancer Res, 2018, 78(20):5731-5740]	PubMed: 30135193

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.