Isatin

Catalog No.S4717 Batch:S471701

Technical Data

Formula	C₈H₅NO₂
Molecular Weight	147.13	CAS No.	91-56-5
Solubility (25°C)*	In vitro	DMSO	29 mg/mL (197.1 mM)
		Ethanol	10 mg/mL (67.96 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Isatin (2,3-Indolinedione) is an endogenous MAO inhibitor with an IC50 of 15 μM and more active against MAO-B than MAO-A.

Targets

MAO-B ^[1] (Cell-free assay)	MAO ^[1] (Cell-free assay)	MAO-A ^[1] (Cell-free assay)
14 μM	15 μM	58 μM

In vitro

Isatin induces apoptosis of MCF-7 cells. Bcl-2 expression is decreased and the ratio of Bcl-2 to Bax is significantly decreased by isatin. The mitochondrial transmembrane potential is markedly decreased and the release of cytochrome c into the cytosol is elevated following treatment with isatin. At the same time, caspase-9 and -3 are stimulated, followed by the degradation of ICAD, a caspase-3 substrate. Isatin and its analogs inhibits the proliferation of some cancer cells, including colon HT29, breast MCF-7, lung A549 and melanoma UACC903 cells and is a dual inhibitor of tubulin polymerization and the Akt pathway^[4].

In vivo

Isatin is an endogenous indole that is increased in stress, inhibits monoamine oxidase (MAO) B and improves bradykinesia and striatal dopamine levels in rat models of Parkinson's disease. Isatin has a distinct and discontinuous distribution in rat brain and other tissues; the highest concentrations in the brain are found in the hippocampus and cerebellum. In rodent models isatin has been shown to cause a widespectrum of dose-dependent physiological and biological actions, such as anxiogenic and sedative effects, memory dysfunction and inhibition of food and water intake. Significantly, isatin readily crosses the blood-brain barrier so that a peritoneal dose of 100 mg/kg would result in a concentration of about 120 μM in the rat brain. This concentration would increase further with repeated injections^[2].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines human neuroblastoma (SH-SY5Y) cells Concentrations 0.1-400 μM Incubation Time 24 or 48 h Method Cell viability was estimated by a colorimetric method, which is based on the ability of cellular dehydrogenases of viable cells to reduce MTT from a yellow watersoluble dye to a dark blue insoluble formazan product. SHSY5Y cells were seeded in 96-well plates at 4×104 cells/well/(100 ml) and allowed to attach. The cells were then treated with isatin and returned to the incubator for 24 or 48 h. MTT 25 μl (5 mg/ml) was added to all wells and allowed to incubate in the dark at 37℃ for 2 h followed by cell lysis. The plates were read with an OPTImax microplate reader at wavelength of 562 nm. Controls included untreated cells and medium alone, with all MTT assays performed in triplicate.
Animal Study:^{^[3]}	Animal Models Albino rats of the Fisher strain Dosages 100 mg/kg Administration i.p

Cell Assay:^{^[2]}

Cell lines
human neuroblastoma (SH-SY5Y) cells
Concentrations
0.1-400 μM
Incubation Time
24 or 48 h
Method
Cell viability was estimated by a colorimetric method, which is based on the ability of cellular dehydrogenases of viable cells to reduce MTT from a yellow watersoluble dye to a dark blue insoluble formazan product. SHSY5Y cells were seeded in 96-well plates at 4×104 cells/well/(100 ml) and allowed to attach. The cells were then treated with isatin and returned to the incubator for 24 or 48 h. MTT 25 μl (5 mg/ml) was added to all wells and allowed to incubate in the dark at 37℃ for 2 h followed by cell lysis. The plates were read with an OPTImax microplate reader at wavelength of 562 nm. Controls included untreated cells and medium alone, with all MTT assays performed in triplicate.

Animal Study:^{^[3]}

Animal Models
Albino rats of the Fisher strain
Dosages
100 mg/kg
Administration
i.p

References

[4] Ma Z, et al. Oncol Rep. 2014, 32(5):2111-7.

+ Expand

Selleck's Isatin has been cited by 1 publication

Genome-wide CRISPR-Cas9 screening identifies the CYTH2 host gene as a potential therapeutic target of influenza viral infection [ Cell Rep, 2022, 38(13):110559]	PubMed: 35354039

Genome-wide CRISPR-Cas9 screening identifies the CYTH2 host gene as a potential therapeutic target of influenza viral infection [ Cell Rep, 2022, 38(13):110559]

PubMed: 35354039

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SHIPPING AND STORAGE
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