Histamine

Catalog No.S3968 Batch:S396802

Technical Data

Formula	C₅H₉N₃
Molecular Weight	111.15	CAS No.	51-45-6
Solubility (25°C)*	In vitro	DMSO	22 mg/mL (197.93 mM)
		Water	22 mg/mL (197.93 mM)
		Ethanol	22 mg/mL (197.93 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Histamine, an organic nitrogenous compound, is involved in local immune responses regulating physiological function in the gut and acting as a neurotransmitter for the brain, spinal cord, and uterus. It is a potent H1 and H2 receptor agonist.
In vitro	Histamine stimulates the proliferation of human articular chondrocytes in culture. Histamine is reported to modify the behaviour of many cell types in vitro, including chondrocytes, fibroblasts, macrophages, epithelial cells, endothelial cells, and T cells. Histamine also modulates the production of many cytokines and the expression of their receptors. Histamine regulates cellular processes through the expression of histamine receptors. Histamine stimulates the production of matrix metalloproteinases (MMPs)-13 and -3 (collagenase 3 and stromelysin-1, respectively) by histidine decarboxylase (HAC) in vitro^[1].
In vivo	Histamine has a recognised role in allergic and inflammatory reactions and is an important modulator of numerous physiological processes, including cell proliferation, angiogenesis, and vasopermeability^[1]. Histamine dilates vasculature, increases blood flow, while it induces hyperpermeability in venula. It disrupts endothelial barrier formation of venula indicated by changes in vascular endothelial cadherin (VE-cadherin) localization at endothelial cell junction^[3].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines human articular chondrocytes Concentrations 1-100 μmol/l Incubation Time 6 days Method Cells are grown to confluence in 80 cm² culture flasks in Dulbecco's modified Eagle's medium (DMEM) + 10% fetal calf serum (FCS). At first or second passage, cells are seeded into 96 well culture plates, at densities of approximately 2×10³ cells/well. After 24 hours the cells are treated with either DMEM +2% FCS alone (control), or DMEM + 2% FCS plus histamine range 1-100 μmol/l (minimum of eight wells for each treatment). The medium with or without histamine is replenished every 48 hours, and after six days cell growth is estimated.
Animal Study:^{^[2]}	Animal Models New Zealand adult healthy albino rabbits Dosages 50, 100, 200 μg/kg Administration s.c.

Cell Assay:^{^[1]}

Cell lines
human articular chondrocytes
Concentrations
1-100 μmol/l
Incubation Time
6 days
Method
Cells are grown to confluence in 80 cm² culture flasks in Dulbecco's modified Eagle's medium (DMEM) + 10% fetal calf serum (FCS). At first or second passage, cells are seeded into 96 well culture plates, at densities of approximately 2×10³ cells/well. After 24 hours the cells are treated with either DMEM +2% FCS alone (control), or DMEM + 2% FCS plus histamine range 1-100 μmol/l (minimum of eight wells for each treatment). The medium with or without histamine is replenished every 48 hours, and after six days cell growth is estimated.

Animal Study:^{^[2]}

Animal Models
New Zealand adult healthy albino rabbits
Dosages
50, 100, 200 μg/kg
Administration
s.c.

References

Selleck's Histamine has been cited by 1 publication

Benidipine, an anti-hypertensive drug, relaxes mouse airway smooth muscle [ Life Sci, 2019, 227:74-81]	PubMed: 31002920

Benidipine, an anti-hypertensive drug, relaxes mouse airway smooth muscle [ Life Sci, 2019, 227:74-81]

PubMed: 31002920

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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