GSH (Glutathione)

Catalog No.S4606 Batch:S460601

Technical Data

Formula	C₁₀H₁₇N₃O₆S
Molecular Weight	307.32	CAS No.	70-18-8
Solubility (25°C)*	In vitro	Water	61 mg/mL (198.49 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	GSH (Glutathione) acts as an antioxidant, a free radical scavenger and a detoxifying agent. It is a tripeptide comprised of three amino acids (cysteine, glutamic acid, and glycine) present in most mammalian tissue.
In vitro	Glutathione (GSH) is able to recover the meiotic failure induced by heavy metal cadmium (Cd) exposure through suppressing the excessive ROS level, DNA damage accumulation and apoptotic incidence in porcine oocytes.^[2]
In vivo	Oral GSH can increase GSH concentrations in several tissues following GSH depletion.^[1]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines porcine oocytes Concentrations 1, 2 and 4 mM Incubation Time -- Method Cadmium chloride is dissolved in PBS and diluted in TCM-199 medium to a final concentration of 0.4, 2, 10 and 50 μM, respectively. GSH is dissolved in PBS and diluted in TCM-199 medium to a final concentration of 1, 2 and 4 mM, respectively. Both cadmium chloride and GSH are supplemented to TCM-199 medium immediately before culture.
Animal Study:^{^[1]}	Animal Models Mice (outbred albino ICR, 30-40 g) Dosages 100 mg/kg Administration oral administration

Cell Assay:^{^[2]}

Cell lines
porcine oocytes
Concentrations
1, 2 and 4 mM
Incubation Time
--
Method
Cadmium chloride is dissolved in PBS and diluted in TCM-199 medium to a final concentration of 0.4, 2, 10 and 50 μM, respectively. GSH is dissolved in PBS and diluted in TCM-199 medium to a final concentration of 1, 2 and 4 mM, respectively. Both cadmium chloride and GSH are supplemented to TCM-199 medium immediately before culture.

Animal Study:^{^[1]}

Animal Models
Mice (outbred albino ICR, 30-40 g)
Dosages
100 mg/kg
Administration
oral administration

References

Selleck's GSH (Glutathione) has been cited by 16 publications

Intermittent dietary methionine deprivation facilitates tumoral ferroptosis and synergizes with checkpoint blockade [ Nat Commun, 2023, 14(1):4758]	PubMed: 37553341
Intermittent dietary methionine deprivation facilitates tumoral ferroptosis and synergizes with checkpoint blockade [ Nat Commun, 2023, 14(1):4758]	PubMed: 37553341
Piperlongumine conquers temozolomide chemoradiotherapy resistance to achieve immune cure in refractory glioblastoma via boosting oxidative stress-inflamation-CD8+-T cell immunity [ J Exp Clin Cancer Res, 2023, 42(1):118]	PubMed: 37161450
Cancer cells reprogram to metastatic state through the acquisition of platelet mitochondria [ Cell Rep, 2023, 42(9):113147]	PubMed: 37756158
Heat shock protein 27 deficiency promotes ferrous ion absorption and enhances acyl-Coenzyme A synthetase long-chain family member 4 stability to promote glioblastoma cell ferroptosis [ Cancer Cell Int, 2023, 23(1):5]	PubMed: 36639654
Glutathione promotes the synergistic effects of venetoclax and azacytidine against myelodysplastic syndrome‑refractory anemia by regulating the cell cycle [ Exp Ther Med, 2023, 26(6):574]	PubMed: 38023359
Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis [ Cell Stem Cell, 2022, 29(7):1119-1134.e7]	PubMed: 35803229
Targeting APLN/APJ restores blood-testis barrier and improves spermatogenesis in murine and human diabetic models [ Nat Commun, 2022, 13(1):7335]	PubMed: 36443325
Unraveling the differential impact of PAHs and dioxin-like compounds on AKR1C3 reveals the EGFR extracellular domain as a critical determinant of the AHR response [ Environ Int, 2022, 158:106989]	PubMed: 34991250
HIF-α activation by the prolyl hydroxylase inhibitor roxadustat suppresses chemoresistant glioblastoma growth by inducing ferroptosis [ Cell Death Dis, 2022, 13(10):861]	PubMed: 36209275

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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