Gentisic acid

Catalog No.S3799 Batch:S379901

Print

Technical Data

Formula

C7H6O4

Molecular Weight 154.12 CAS No. 490-79-9
Solubility (25°C)* In vitro DMSO 30 mg/mL (194.65 mM)
Water 7.5 mg/mL (48.66 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Gentisic acid (2,5-dihydroxybenzoic acid, 5-Hydroxysalicylic acid, Hydroquinonecarboxylic acid, DHB), an active metabolite of salicylic acid degradation, has a broad spectrum of biological activity, such as anti-inflammatory, antirheumatic and antioxidant properties.
In vitro

Gentisic acid (GA) has been shown to act as an antioxidant under different types of physical and chemical stimulus. Gentisic acid efficiently protects lipids, proteins, SOD enzyme and human erythrocytes on gamma radiation exposure. It has been found to scavenge physiologically relevant hydroxyl and peroxyl radicals in pulse radiolysis studies[3].

In vivo

Gentisic acid (GA) shows a potent protective effect against CP induced hepatotoxicity and genotoxicity. The protective effect of Gentisic acid can be attributed to its antioxidant properties, which enhance natural antioxidant enzymes and the cell membrane stabilizing property by inhibiting lipid peroxidation[2].

Protocol (from reference)

Cell Assay:

[1]

  • Cell lines

    HTC cells

  • Concentrations

    0.08, 0.16, 0.8, 1.6, and 8 μg/mL

  • Incubation Time

    24 h

  • Method

    The Trypan Blue exclusion protocol is assessed in 24-well cell culture plates. In each well, 2.5×cells are seeded in 2 mL of complete culture medium with the following treatments: control (CO, 50 μL of culture medium, as negative control), cytotoxic agent doxorubicin (DXR, 10 μg/mL, as positive control), and Gentisic acid (0.08, 0.16, 0.8, 1.6, and 8 μg/mL). The cells are incubated for 24 h, trypsinized and then resuspended in 0.5 mL of culture medium. An aliquot of the cellular suspension (20 μL) is homogenized with Trypan Blue (0.2%) dye in a 1:1 ratio. The cells are quantified with a Neubauer chamber hemocytometer.

Animal Study:

[2]

  • Animal Models

    male Swiss albino mice

  • Dosages

    50 and 100 mg/kg

  • Administration

    oral administration

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.