Gallic acid

Catalog No.S4603 Batch:S460301

Technical Data

Formula	C₇H₆O₅
Molecular Weight	170.12	CAS No.	149-91-7
Solubility (25°C)*	In vitro	DMSO	34 mg/mL (199.85 mM)
		Ethanol	34 mg/mL (199.85 mM)
		Water	7 mg/mL (41.14 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Gallic acid (3,4,5-Trihydroxybenzoic acid, Gallate, Benzoic acid), known as powerful antioxidants, is an organic acid found in a variety of foods and herbs.
In vitro	Gallic acid does not protect against H(2)O(2)-induced PC12 cell death. It reduced the viability of PC12 cells in a dose-dependent manner. Gallic acid also induces cleavage of poly (ADP-ribose) polymerase, which is strongly related to apoptosis in neurons. Gallic acid induces the phosphorylation of c-Jun N-terminal protein kinase (JNK) and the downregulation of Bcl-2 in PC12 cells. Gallic acid leads to a progressive reduction in the viability of vector-transfected PC12 cells, which is delayed in PC12 cells that overexpressed Bcl-2^[2].
In vivo	Gallic acid effectively protects rat erythrocytes. The antioxidant effect of the compound at the tested dosage in vivo was more prevalent than its prooxidative effects. Gallic acid has ameliorative effect on lipid peroxidation in vivo^[1].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines PC12 cells Concentrations 25 or 50 μM Incubation Time 24 h Method Cell viability is measured using the MTT assay. PC12 cells are incubated at 37◦C with H2O2 or gallic acid at different concentrations for 24 h with or without pretreatment with resveratrol. They are then treated with the MTT solution (final concentration, 1 mg/mL) for 2 h. The dark-blue formazan crystals that formed in intact cells are dissolved in DMSO, and the absorbance at 570 nm is measured using a micro-ELISA reader.
Animal Study:^{^[1]}	Animal Models Male Wistar rats Dosages 10 mg/kg and 20 mg/kg Administration i.p.

Cell Assay:^{^[2]}

Cell lines
PC12 cells
Concentrations
25 or 50 μM
Incubation Time
24 h
Method
Cell viability is measured using the MTT assay. PC12 cells are incubated at 37◦C with H2O2 or gallic acid at different concentrations for 24 h with or without pretreatment with resveratrol. They are then treated with the MTT solution (final concentration, 1 mg/mL) for 2 h. The dark-blue formazan crystals that formed in intact cells are dissolved in DMSO, and the absorbance at 570 nm is measured using a micro-ELISA reader.

Animal Study:^{^[1]}

Animal Models
Male Wistar rats
Dosages
10 mg/kg and 20 mg/kg
Administration
i.p.

References

Selleck's Gallic acid has been cited by 3 publications

Single-cell profiling reveals a potent role of quercetin in promoting hair regeneration [ Protein Cell, 2023, 14(6):398-415]	PubMed: 37285263
IDH2 stabilizes HIF-1α-induced metabolic reprogramming and promotes chemoresistance in urothelial cancer [ EMBO J, 2023, e110620.]	PubMed: 36637036
Resveratrol compounds inhibit human holocarboxylase synthetase and cause a lean phenotype in Drosophila melanogaster. [ J Nutr Biochem, 2015, 26(11):1379-84]	PubMed: 26303405

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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