FIN56

Catalog No.S8254 Batch:S825401

Technical Data

Formula	C₂₅H₃₁N₃O₅S₂
Molecular Weight	517.66	CAS No.	1083162-61-1
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (193.17 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

FIN56 is a specific inducer of ferroptosis .

Targets

Ferroptosis ^[1]

In vitro

FIN56 is a specific inducer of ferroptosis. The mechanism involves two distinct pathways: one pathway is degradation of GPX4, which requires the enzymatic activity of acetyl-CoA carboxylase; the other pathway is activation of squalene synthase, which leads to coenzyme Q10 depletion, in a manner independent of GPX4 degradation^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HT-1080 cells Concentrations 5 μM Incubation Time 10 h Method 500,000 HT-1080 cells are seeded in a 10-cm dish. Cells are grown at 37 °C for 16 h. On the day of the analysis, cells are cotreated with 100 μM α-tocopherol and either vehicle (DMSO) or a ferroptosis inducer (10 μM erastin, 0.5 μM (1S, 3R)-RSL3, or 5 μM FIN56) and incubated for 10 h. Cells are then trypsinized, pelleted, washed once with 400 μL of ice-cold PBS containing 1 mM EDTA, and sonicated. After the cell debris have been pelleted and removed, both oxidized and reduced glutathione in 120 μL of sample is quantified in technical triplicates. The glutathione quantity is normalized to the protein concentration measured via Bradford assay.

Cell Assay:^{^[1]}

Cell lines
HT-1080 cells
Concentrations
5 μM
Incubation Time
10 h
Method
500,000 HT-1080 cells are seeded in a 10-cm dish. Cells are grown at 37 °C for 16 h. On the day of the analysis, cells are cotreated with 100 μM α-tocopherol and either vehicle (DMSO) or a ferroptosis inducer (10 μM erastin, 0.5 μM (1S, 3R)-RSL3, or 5 μM FIN56) and incubated for 10 h. Cells are then trypsinized, pelleted, washed once with 400 μL of ice-cold PBS containing 1 mM EDTA, and sonicated. After the cell debris have been pelleted and removed, both oxidized and reduced glutathione in 120 μL of sample is quantified in technical triplicates. The glutathione quantity is normalized to the protein concentration measured via Bradford assay.

References

[1] Shimada K, et al. Nat Chem Biol. 2016, 12(7):497-503.

Selleck's FIN56 has been cited by 24 publications

PKCiota Inhibits the Ferroptosis of Esophageal Cancer Cells via Suppressing USP14-Mediated Autophagic Degradation of GPX4 [ Antioxidants (Basel), 2024, 13(1)114]	PubMed: 38247539
The cell fate regulator DACH1 modulates ferroptosis through affecting P53/SLC25A37 signaling in fibrotic disease [ Hepatol Commun, 2024, 8(3)e0396]	PubMed: 38437058
Myeloid-like tumor hybrid cells in bone marrow promote progression of prostate cancer bone metastasis [ J Hematol Oncol, 2023, 16(1):46]	PubMed: 37138326
Targeting NRF2 uncovered an intrinsic susceptibility of acute myeloid leukemia cells to ferroptosis [ Exp Hematol Oncol, 2023, 12(1):47]	PubMed: 37198609
Selective ablation of primary and paracrine senescent cells by targeting iron dyshomeostasis [ Cell Rep, 2023, 42(2):112058]	PubMed: 36753419
MMD collaborates with ACSL4 and MBOAT7 to promote polyunsaturated phosphatidylinositol remodeling and susceptibility to ferroptosis [ Cell Rep, 2023, 42(9):113023]	PubMed: 37691145
Dihydroartemisinin-induced ferroptosis in acute myeloid leukemia: links to iron metabolism and metallothionein [ Cell Death Discov, 2023, 9(1):97]	PubMed: 36928207
Ferroptosis heterogeneity in triple-negative breast cancer reveals an innovative immunotherapy combination strategy [ Cell Metab, 2022, S1550-4131-2200411-9]	PubMed: 36257316
ACSL4-dependent ferroptosis does not represent a tumor-suppressive mechanism but ACSL4 rather promotes liver cancer progression [ Cell Death Dis, 2022, 13(8):704]	PubMed: 35963845
Induction of autophagy-dependent ferroptosis to eliminate drug-tolerant human retinoblastoma cells [ Cell Death Dis, 2022, 13(6):521]	PubMed: 35654783

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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