Evans Blue

Catalog No.S4716 Batch:S471601

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Technical Data

Formula

C34H24N6O14S4.4Na
Molecular Weight 960.81 CAS No. 314-13-6
Solubility (25°C)* In vitro DMSO 100 mg/mL (104.07 mM)
Water 100 mg/mL (104.07 mM)
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Evans Blue (Direct Blue 53,C.I.23860) is a potent inhibitor of the uptake of L-glutamate into synaptic vesicles, also an AMPA/kainate receptor antagonist.
Targets
AMPA/kainate receptor [7] vesicular glutamate uptake [5]
87 nM
In vitro

Evans Blue is found to be inhibiting DNA binding of NF-κB at a low concentration of 100 μM[1]. It has proven over the years to be a dependable stain for microscopic determination of cell death[2]. Evans Blue is also a known blocker of a subset of a-amino-3-hydroxy-5-methyl-isoxazole/kainate receptors (IC50=355 nM) for the subunit combination GluR1,2[5]. Evans blue is the first known δ-subunit-specific antagonist of ENaC and activates large-conductance Ca2+-activated K+ channels in sheep bladder myocytes and cultured endothelial cells of human umbilical veins[5].
In vivo

Evans blue pretreatment could inhibit the mast cells degranulation and that may be the main mechanism for prevent animals from C48/80-triggered anaphylaxis and systemic inflammation[4]. Evans blue modulates the non-N-methyl-d-aspartate receptor in rat thalamic neurons, blocks the P2X-purinergic receptor in rat vas deferens, and inhibits the glutamate transporter in rat brain synaptic vesicles[6].

Protocol (from reference)

Animal Study:

[3]
  • Animal Models

    CD hairless rats (Charles River)

  • Dosages

    100 mg/kg

  • Administration

    i.p

Selleck's Evans Blue has been cited by 1 publication

Toll-like receptor 4 and lipopolysaccharide from commensal microbes regulate Tembusu virus infection [ J Biol Chem, 2022, 298(12):102699] PubMed: 36379254

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.