Ethyl ferulate

Catalog No.S3855 Batch:S385501

Technical Data

Formula	C₁₂H₁₄O₄
Molecular Weight	222.24	CAS No.	4046-02-0
Solubility (25°C)*	In vitro	DMSO	44 mg/mL (197.98 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Ethyl ferulate (Ferulic acid ethyl ester) is the alkyl ester derivative of ferulic acid which is a naturally occurring plant product with anti-oxidative, anti-inflammatory, neuroprotective, and antiproliferative activities.
In vitro	Ethyl ferulate is an inducer of HO-1 expression, at both mRNA and protein levels, and HO activity in cultured astrocytes and hippocampal neurons^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Rat type 1 astrocytes (DI TNC1) and rat hippocampal neurons (H 19-7) Concentrations 0-25 μM Incubation Time 6 or 24 h Method Cells are grown in 75-cm² flasks and maintained at 37℃ in a humidified atmosphere of air and 5% CO2. Confluent cells are exposed to various concentrations of EFE and RSV. After each treatment (6 or 24 h), cells are harvested for the determination of HO activity, HO-1 and HO-2 mRNA levels, and protein expression. Neurons growing in 24 wells are also exposed to different concentrations of EFE, and cell viability is determined at 24 h. Other neurons are pretreated for 12 h with 5 µM EFE or 5 µM EFE plus 10 µM ZnPP IX and then exposed for 2 h to 50 mU/mL GOX, before cell viability is determined.

Cell Assay:^{^[1]}

Cell lines
Rat type 1 astrocytes (DI TNC1) and rat hippocampal neurons (H 19-7)
Concentrations
0-25 μM
Incubation Time
6 or 24 h
Method
Cells are grown in 75-cm² flasks and maintained at 37℃ in a humidified atmosphere of air and 5% CO2. Confluent cells are exposed to various concentrations of EFE and RSV. After each treatment (6 or 24 h), cells are harvested for the determination of HO activity, HO-1 and HO-2 mRNA levels, and protein expression. Neurons growing in 24 wells are also exposed to different concentrations of EFE, and cell viability is determined at 24 h. Other neurons are pretreated for 12 h with 5 µM EFE or 5 µM EFE plus 10 µM ZnPP IX and then exposed for 2 h to 50 mU/mL GOX, before cell viability is determined.

References

[1] Scapagnini G, et al. Antioxid Redox Signal. 2004, 6(5):811-8.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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