EC330

Catalog No.S0472 Batch:S047201

Technical Data

Formula	C₃₀H₃₂F₂O₂
Molecular Weight	462.57	CAS No.	2016795-77-8
Solubility (25°C)*	In vitro	Ethanol	93 mg/mL (201.05 mM)
		DMSO	12 mg/mL (25.94 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

EC330 is a steroidal inhibitor of leukemia inhibitory factor (LIF, an interleukin 6 class cytokine). EC330 inhibits the LIF/LIF-R signaling and blocks the promoting effects of LIF on growth and migration of cancer cells.

Targets

LIF ^[1]

In vitro

EC330, a potential LIF inhibitor, inhibits the activation of STAT3, PI3K/AKT, and mTOR signaling pathways by LIF, preferentially inhibits the proliferation of cancer cells with LIF overexpression, also inhibits LIF function in promoting migration of cancer cells.^[1]

In vivo

EC330 inhibits multi-functional cytokine LIF function in promoting tumor growth in vivo.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines MCF7, MDA-MB231 cell lines Concentrations -- Incubation Time 24 h Method The effect of EC330 on viability of cells with or without ectopic LIF expression was assessed in cells treated with different concentrations of EC330 for 24 h. Cell viability was determined by the Vi-CELL counter that performs a trypan blue exclusion method. Cell proliferation was determined by counting cell numbers daily for four days.
Animal Study:^{^[1]}	Animal Models BALB/c nude mice model of subcutaneous xenograft tumors formed by MDA-MB231 cells with ectopic LIF expression Dosages 1 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
MCF7, MDA-MB231 cell lines
Concentrations
--
Incubation Time
24 h
Method
The effect of EC330 on viability of cells with or without ectopic LIF expression was assessed in cells treated with different concentrations of EC330 for 24 h. Cell viability was determined by the Vi-CELL counter that performs a trypan blue exclusion method. Cell proliferation was determined by counting cell numbers daily for four days.

Animal Study:^{^[1]}

Animal Models
BALB/c nude mice model of subcutaneous xenograft tumors formed by MDA-MB231 cells with ectopic LIF expression
Dosages
1 mg/kg
Administration
i.p.

References

[1] Xuetian Yue, et al. J Mol Cell Biol. 2020 Jul 3;12(6):477-480.

Selleck's EC330 has been cited by 1 publication

PCK1 regulates neuroendocrine differentiation in a positive feedback loop of LIF/ZBTB46 signalling in castration-resistant prostate cancer [ Br J Cancer, 2021, 10.1038/s41416-021-01631-3]	PubMed: 34815524

PCK1 regulates neuroendocrine differentiation in a positive feedback loop of LIF/ZBTB46 signalling in castration-resistant prostate cancer [ Br J Cancer, 2021, 10.1038/s41416-021-01631-3]

PubMed: 34815524

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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