Durvalumab (anti-PD-L1)

Catalog No.A2013        Batch: A201305

Print

Technical Data

CAS No. 1428935-60-7
Formulation PBS buffer, pH 7.2
Isotype Human IgG1
Source CHO cells
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 99%
Protein concentration 5.87mg/ml
Endotoxin Level ＜1EU/mg

Biological Activity

Description Durvalumab (anti-PD-L1) is a selective, high affinity human IgG1 mAb that blocks programmed cell death ligand-1 (PD-L1) binding to PD-1 (IC50=0.1 nM) and CD80 (IC50=0.04 nM). MW=146.3 kDa.
Targets
PD-L1/CD80 [1]
(HTRF assay)		 PD-1/PD-L1 interaction [1]
(HTRF assay)
0.04 nM 0.1 nM
In vitro

The labeled radioligand shows good affinity to high PD-L1 expression cells and could be blocked with excess unlabeled intact durvalumab.[1]
In vivo

The peak tumor uptake of 124I-Durva-F(ab’)2 was close to 124I-Durva, but much earlier (5.29±0.42% ID/g for 124I-Durva-F(ab’)2 at 12 h vs 5.18±0.73% ID/g for 124I-Durva at 48 h). Compared with 124I-Durva, an accelerated blood clearance was observed for 124I-Durva-F(ab’)2, allowing for a higher tumor-to-background ratio.[1]

Protocol (Only for Reference)

Cell Assay:

  • The A549 and H460 cells were cultured in six-well plates (~106 cells/well) and divided into two groups. All of the wells were incubated with 5% BSA for blocking nonspecific binding. Group A was presaturated with 100-fold excess of unlabeled durvalumab to block the binding of 124I-Durva-F(ab′)2, and group B had only the medium added. After 30 min, 2 nM of 124I-Durva-F(ab′)2 was added to each group and incubated for 1 h at 37 °C in a cell incubator. After supernatant collection, the cells were lysed using 2 M of NaOH.
Animal Study:

  • Animal Models: H460 and A549 tumor-bearing mice
    Dosages: 20 μCi
    Administration: i.v.

Customer Product Validation

Selleck's Durvalumab (anti-PD-L1) has been cited by 14 publications

Noninvasive PET imaging of tumor PD-L1 expression with 64Cu-labeled Durvalumab [ Am J Nucl Med Mol Imaging, 2024, 14(1):31-40] PubMed: 38500749
Development of a highly sensitive chemiluminescence immunoassay using a novel signal-enhanced detection system for quantitation of durvalumab, an immune-checkpoint inhibitor monoclonal antibody used for immunotherapy of lung cancer [ Heliyon, 2023, 9(6):e15782] PubMed: 37389074
Development of a highly sensitive chemiluminescence immunoassay using a novel signal-enhanced detection system for quantitation of durvalumab, an immune [ Heliyon, 2023, 9(6):e15782.] PubMed: 37389074
Characterization of INCB086550: A Potent and Novel Small-Molecule PD-L1 Inhibitor [ Cancer Discov, 2022, 12(6):1482-1499] PubMed: 35254416
Characterization of INCB086550: A Potent and Novel Small-Molecule PD-L1 Inhibitor [ Cancer Discov, 2022, 12(6):1482-1499] PubMed: 35254416
SBSN drives bladder cancer metastasis via EGFR/SRC/STAT3 signalling [ J Med Chem, 2022, 65(5):3879-3893] PubMed: 35188766
Sunitinib potentiates the cytotoxic effect of electrochemotherapy in pancreatic carcinoma cells [ Mol Pharm, 2022, 10.1021/acs.molpharmaceut.2c00084] PubMed: 35244407
Programmed death ligand-1 regulates angiogenesis and metastasis by participating in the c-JUN/VEGFR2 signaling axis in ovarian cancer [ Cancer Commun (Lond), 2021, 41(6):511-527] PubMed: 33939321
Novel Biphenyl Pyridines as Potent Small-Molecule Inhibitors Targeting the Programmed Cell Death-1/Programmed Cell Death-Ligand 1 Interaction [ J Med Chem, 2021, 64(11):7390-7403] PubMed: 34056906
Galvanotactic Migration of Glioblastoma and Brain Metastases Cells [ J Med Chem, 2021, 64(15):11614-11636] PubMed: 34313116

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

Specific storage and handling information for each product is indicated on the product datasheet. Most Selleck products are stable under the recommended conditions. Products are sometimes shipped at a temperature that differs from the recommended storage temperature. Short-term storage of many products are stable in the short-term at temperatures that differ from that required for long-term storage.
We ensure that the product is shipped under conditions that will maintain the quality of the reagents. Upon receipt of the product, follow the storage recommendations on the product data sheet.