Dibutyryl-cAMP (Bucladesine)

Catalog No.S7858 Batch:S785811

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Technical Data

Formula

C18H23N5NaO8P
Molecular Weight 491.37 CAS No. 16980-89-5
Solubility (25°C)* In vitro DMSO 98 mg/mL (199.44 mM)
Water 98 mg/mL (199.44 mM)
Ethanol 25 mg/mL (50.87 mM)
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
4.9mg/ml Taking the 1 mL working solution as an example, add 50 μL of 98 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Dibutyryl-cAMP (Bucladesine, dbcAMP) is a cell-permeable PKA activator by mimicing the action of endogenous cAMP. Dibutyryl-cAMP (Bucladesine) is also a phosphodiesterase (PDE) inhibitor.
Targets
PDE [5] PKA [1]
In vitro

Dibutyryl-cAMP inhibits neuronal glucose uptake via PKA activation. [1]

In cultured rat hepatocytes, Dibutyryl-cAMP inhibits inducible nitric oxide synthase expression and NF-kappaB-binding activity. [2]

Dibutyryl-cAMP also suppress TNFalpha-induced hepatocyte apoptosis by inhibiting FADD up-regulation. [3]
In vivo

In a mouse model, bucladesine (600 nM/mouse, i.p.) reverses zinc chloride- and lead acetate-induced avoidance memory retention impairments. [4]

Protocol (from reference)

Cell Assay:

[6]
  • Cell lines

    PC12 cells

  • Concentrations

    1 mM

  • Incubation Time

    24 h

  • Method

    Cells were incubated with 1 mM of db-cAMP.
Animal Study:

[4]
  • Animal Models

    Mice

  • Dosages

    600 nM/mouse

  • Administration

    i.p.

Selleck's Dibutyryl-cAMP (Bucladesine) has been cited by 37 publications

Activation of hepatic adenosine A1 receptor ameliorates MASH via inhibiting SREBPs maturation [ Cell Rep Med, 2024, 5(3):101477] PubMed: 38508143
Jiao-tai-wan and its effective component-berberine improve diabetes and depressive disorder through the cAMP/PKA/CREB signaling pathway [ J Ethnopharmacol, 2024, 324:117829] PubMed: 38296172
IL-6-Derived Autocrine Lactate Promotes Immune Escape of Uveal Melanoma [ Invest Ophthalmol Vis Sci, 2024, 65(3):37] PubMed: 38551584
Host-to-graft propagation of inoculated α-synuclein into transplanted human induced pluripotent stem cell-derived midbrain dopaminergic neurons [ Regen Ther, 2024, 25:229-237] PubMed: 38283940
Axonal Lysosomal Assays for Characterizing the Effects of LRRK2 G2019S [ Biology (Basel), 2024, 13(1)58] PubMed: 38275734
Balanced SET levels favor the correct enhancer repertoire during cell fate acquisition [ Nat Commun, 2023, 14(1):3212] PubMed: 37270547
Probabilistic cell seeding and non-autofluorescent 3D-printed structures as scalable approach for multi-level co-culture modeling [ Mater Today Bio, 2023, 21:100706] PubMed: 37435551
Elevated high-mannose N-glycans hamper endometrial decidualization [ iScience, 2023, 10.1016/j.isci.2023.108170] PubMed: 37915610
Expression and role of nicotinic acetylcholine receptors during midbrain dopaminergic neuron differentiation from human induced pluripotent stem cells [ Neuropsychopharmacol Rep, 2023, 10.1002/npr2.12361] PubMed: 37366076
Persistent DNA damage associated with ATM kinase deficiency promotes microglial dysfunction [ Nucleic Acids Res, 2022, 50(5):2700-2718] PubMed: 35212385

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.