Deoxycholic acid

Catalog No.S4689 Batch:S468901

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Technical Data

Formula

C24H40O4
Molecular Weight 392.57 CAS No. 83-44-3
Solubility (25°C)* In vitro DMSO 78 mg/mL (198.69 mM)
Ethanol 60 mg/mL (152.83 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO Corn oil
3.9mg/ml Taking the 1 mL working solution as an example, add 50 μL of 78 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Deoxycholic acid (Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic) is a cytolytic agent. The physiologic effect of deoxycholic acid is by means of decreased cell membrane integrity. Deoxycholic acid is specifically responsible for activating the G protein-coupled bile acid receptor TGR5 that stimulates brown adipose tissue (BAT) thermogenic activity.
In vitro Deoxycholic acid inhibits miR-21 expression in primary rat hepatocytes in a dose-dependent manner, and increases miR-21 pro-apoptotic target programmed cell death 4 (PDCD4) and apoptosis. Deoxycholic acid decreases NF-κB activity, shown to represent an upstream mechanism leading to modulation of the miR-21/PDCD4 pathway[1].
In vivo miR-21 expression is down-regulated upon short exposures to Deoxycholic acid, as is the downstream pathway, with concomitant PIDD processing and activation of caspase-2, which contributes to DCA-induced liver damage[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Primary rat hepatocytes

  • Concentrations

    25 to 200 μM

  • Incubation Time

    24 h

  • Method

    Primary rat hepatocytes were isolated from male rats (100 to 150 g) by collagenase perfusion. After isolation, hepatocytes were resuspended in complete Williams E medium and plated on BD Primaria™ culture dishes at 5 × 104 cells/cm2. Cells are kept at 37 °C in a humidified atmosphere of 5% CO2 for 4-6 h to allow attachment. Plates are then washed with phosphate buffered saline (PBS) 1× in order to remove dead cells and incubated in Williams E medium supplemented with 25 to 200 μM DCA or no addition (control) for 24 h. Primary rat hepatocytes are processed for total RNA and protein isolation, cell viability, cytotoxicity and caspase activity assays and Hoechst staining.
Animal Study:

[1]
  • Animal Models

    Male Wistar rats

  • Dosages

    125 mg/kg

  • Administration

    p.o.

Selleck's Deoxycholic acid has been cited by 1 publication

Analysis of the Ubiquitination and Phosphorylation of Vangl Proteins [ Bio Protoc, 2022, 12(20)e4533] PubMed: 36353719

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.