Crocin

Catalog No.S4758 Batch:S475801

Technical Data

Formula	C₄₄H₆₄O₂₄
Molecular Weight	976.96	CAS No.	42553-65-1
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (102.35 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Crocin (Alpha-Crocin, Gardenia Yellow) is a water-soluble carotenoid pigment of saffron (Crocus sativus L.). It has been used as a spice for flavoring and coloring food preparations, and in Chinese traditional medicine as an anodyne or tranquilizer.
In vitro	Crocin can significantly inhibit the proliferation of MCF-7 cells, and induce their apoptosis through mitochondrial signaling pathways including the activation of Caspase-8, upregulation of Bax, the disruption of mitochondrial membrane potential (MMP), and the release of cytochrome c^[1]. Crocin decreases cell viability in DLA (Daltons lymphoma) cells, in a concentration- and time-dependent manner^[2].
In vivo	Crocin exhibits a variety of pharmacological effects in mice including inhibition of skin tumor growth, improvement of learning behavior previously impaired by ethanol, anti-hyperlipidemic effects, therapeutic efficacy for colon adenocarcinomas in rats, anti-atherosclerotic effects and anti-oxidant effects in PC-12 cells by increasing GSH synthesis. For Daltons lymphoma models, The lifespan of ascities tumor bearing mice treated with crocin, are found to be significantly increasing and a significant reduction in solid tumour volume is found in crocin treated animals when compared to that of control animals. Crocin also shows significant impact on hematological parameters, like the hemoglobin count and numbers of lymphocytes^[2]. It has shown that crocin exhibits beneficial effects on many organs including the nervous system, the most studied, gastrointestinal, cardiovascular, genital, endocrine, immune systems, etc. At pharmacological doses it does not harm liver and other organs' function^[3].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines MCF-7 cells Concentrations 10, 25, 50 µg/ml Incubation Time 24 h Method MCF-7 cells were treated with 10, 25, 50 µg/ml Crocin for 24 h and washed with PBS buffer, then fixed in ice-cold 70% ethanol for 15 min. A suspension of 100 µL was taken and incubated with 5 µL propidium iodine (20 µg/ml) and 5 µL annexin V-FITC in the dark for 30 min at room temperature. Finally, 400 µL cold PBS was added to each tube and analyzed by flow cytometry.
Animal Study:^{^[1]}	Animal Models Swiss albino mice injected 1 × 10⁶ Daltons lymphoma cells to the peritoneal cavity Dosages 10 ml/kg Administration oral

Cell Assay:^{^[1]}

Cell lines
MCF-7 cells
Concentrations
10, 25, 50 µg/ml
Incubation Time
24 h
Method
MCF-7 cells were treated with 10, 25, 50 µg/ml Crocin for 24 h and washed with PBS buffer, then fixed in ice-cold 70% ethanol for 15 min. A suspension of 100 µL was taken and incubated with 5 µL propidium iodine (20 µg/ml) and 5 µL annexin V-FITC in the dark for 30 min at room temperature. Finally, 400 µL cold PBS was added to each tube and analyzed by flow cytometry.

Animal Study:^{^[1]}

Animal Models
Swiss albino mice injected 1 × 10⁶ Daltons lymphoma cells to the peritoneal cavity
Dosages
10 ml/kg
Administration
oral

References

Selleck's Crocin has been cited by 2 publications

Crocin induces ROS-mediated papillary thyroid cancer cell apoptosis by modulating the miR-34a-5p/PTPN4 axis in vitro [ Toxicol Appl Pharmacol, 2022, 437:115892]	PubMed: 35085590
Rapid and Prolonged Antidepressant-like Effect of Crocin Is Associated with GHSR-Mediated Hippocampal Plasticity-related Proteins in Mice Exposed to Prenatal Stress. [ ACS Chem Neurosci, 2020, 15;11(8):1159-1170]	PubMed: 32203651

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SHIPPING AND STORAGE
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