CPI-637

Catalog No.S8190 Batch:S819002

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Technical Data

Formula

C22H22N6O

Molecular Weight 386.45 CAS No. 1884712-47-3
Solubility (25°C)* In vitro DMSO 26 mg/mL (67.27 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description CPI-637 is a selective and cell-active benzodiazepinone CBP/EP300 bromodomain inhibitor with IC50 values of 0.03 μM and 0.051μM for CBP and EP300 respectively in TR-FRET assay. It is highly selective against other bromodomains, displaying substantial biochemical activity only against BRD9.
Targets
CBP [1]
(Cell-free assay)
EP300 [1]
(Cell-free assay)
0.03 μM 0.051 μM
In vitro CPI-637 inhibits MYC expression in AMO-1 cells (EC50=0.60 μM)[1].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    AMO-1 cells

  • Concentrations

    0-5 μM

  • Incubation Time

    6 h

  • Method

    AMO-1 cells (DSMZ) were plated at 20,000 cells/well in 96-well plates in Iscove's Modified Dulbecco's Medium supplemented with 10% fetal calf serum. Cells were treated for 6 h with a dose titration of CPI-637 starting from a 5 µM top concentration. After 6 h, cells were lysed and processed for QuantiGene Plex expression analysis and data were collected on a MAGPIX multiplex Luminex instrument.

Selleck's CPI-637 has been cited by 2 publications

P300 Interacted With N-Myc and Regulated Its Protein Stability via Altering Its Post-Translational Modifications in Neuroblastoma [ Mol Cell Proteomics, 2023, 22(3):100504] PubMed: 36708875
Y08197 is a novel and selective CBP/EP300 bromodomain inhibitor for the treatment of prostate cancer. [ Acta Pharmacol Sin, 2019, 10.1038/s41401-019-0237-5] PubMed: 31097763

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.