Ciliobrevin D

Catalog No.S9743 Batch:S974301

Technical Data

Formula

C₁₇H₈Cl₃N₃O₂

Molecular Weight

392.62

CAS No.

1370554-01-0

Solubility (25°C)*

In vitro

DMSO

20 mg/mL (50.93 mM)

Water

Insoluble

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	1.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	0.25mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Ciliobrevin D (compound 5) is a cell-permeable, reversible and specific antagonist of AAA+ (ATPases associated with diverse cellular activities) ATPase motor cytoplasmic dynein. Ciliobrevin D perturbs primary cilia formation and blocks Hedgehog (Hh) signaling.

Targets

cytoplasmic dynein ^[1]

Hedgehog ^[1]

In vitro

Ciliobrevin D inactivate dynein in Sertoli cells. The inactivation of dynein by ciliobrevin D also perturbs gross disruption of F-actin across the Sertoli cells in vitro.^[2] Ciliobrevin D perturbs protein trafficking within the primary cilium, leading to their malformation and Hedgehog signaling blockade. Ciliobrevin D also prevents spindle pole focusing, kinetochore-microtubule attachment, melanosome aggregation, and peroxisome motility in cultured cells.^[1]

In vivo

Ciliobrevin D inactivate dynein in the testis. The inactivation of dynein by ciliobrevin D also perturbs gross disruption of F-actin across the seminiferous epithelium in vivo, illustrating there are cross talks between the two cytoskeletons in the testis.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Sertoli cells Concentrations 15 µM, 30 µM Incubation Time 1 h Method Sertoli cells cultured in vitro on Matrigel-coated bicameral units at 1.0 × 10⁶ cells/cm² are used for quantifying the transepithelial electrical resistance in ohms (Ω) across the cell epithelium to assess the TJ-barrier function. In brief, each bicameral unit is placed inside the well of a 24-well dish with 0.5 ml F12/DMEM in the apical and the basal compartments. Dynein is inhibited in Sertoli cells on days 3 and 4 using Ciliobrevin D at 15 µM or 30 µM for 1hr, and Sertoli cell TJ-permeability barrier function is monitored daily by quantifying transepithelial electrical resistance across the cell epithelium.
Animal Study:^{^[2]}	Animal Models adult male Sprague-Dawley rats Dosages 15 µM per testis Administration local injection

Cell Assay:^{^[2]}

Cell lines
Sertoli cells
Concentrations
15 µM, 30 µM
Incubation Time
1 h
Method
Sertoli cells cultured in vitro on Matrigel-coated bicameral units at 1.0 × 10⁶ cells/cm² are used for quantifying the transepithelial electrical resistance in ohms (Ω) across the cell epithelium to assess the TJ-barrier function. In brief, each bicameral unit is placed inside the well of a 24-well dish with 0.5 ml F12/DMEM in the apical and the basal compartments. Dynein is inhibited in Sertoli cells on days 3 and 4 using Ciliobrevin D at 15 µM or 30 µM for 1hr, and Sertoli cell TJ-permeability barrier function is monitored daily by quantifying transepithelial electrical resistance across the cell epithelium.

Animal Study:^{^[2]}

Animal Models
adult male Sprague-Dawley rats
Dosages
15 µM per testis
Administration
local injection

References

Selleck's Ciliobrevin D has been cited by 2 publications

Localization of a TORC1-eIF4F translation complex during CD8+ T cell activation drives divergent cell fate [ Mol Cell, 2022, S1097-2765(22)00327-6]	PubMed: 35597236
LRRK1-mediated NDEL1 phosphorylation promotes cilia disassembly via dynein-2-driven retrograde intraflagellar transport [ J Cell Sci, 2022, 135(21)jcs259999]	PubMed: 36254578

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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