Chloroquine

Catalog No.S6999 Batch:S699903

Technical Data

Formula	C₁₈H₂₆ClN₃
Molecular Weight	319.87	CAS No.	54-05-7
Solubility (25°C)*	In vitro	DMSO	64 mg/mL (200.08 mM)
		Ethanol	64 mg/mL (200.08 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Chloroquine is an antimalarial drug and autophagy/lysosome inhibitor. Chloroquine also suppresses Toll-like receptor-9 (TLR9) protein expression. Chloroquine is highly effective agianst SARS-CoV-2 (COVID-19) infection with EC50 of 1.13 μM in Vero E6 cells. Chloroquine has anti-HIV-1 activity.

Targets

Autophagy ^[1]	lysosome ^[1]	TLR9 ^[2]	HIV-1 ^[4]	SARS-CoV-2 ^[3] (Vero E6 cells based assay)
				1.13 μM(EC50)

In vitro

Chloroquine is a widely used autophagy inhibitor.^[5]

In vivo

The inhibition of autophagy by chloroquine potentiates the therapeutic effect of PI3K/AKT inhibitors in vitro and in vivo TNBC models, synergizing with taxane-based chemotherapy.^[6]

Protocol (from reference)

Cell Assay:^{^[5]}	Cell lines HL‐60 cells Concentrations 10 µM Incubation Time 48 h Method One day before treatment, AML cells were seeded with a density of 2 × 10⁵ cells/mL. Fresh medium containing different concentrations of Ara‐C was added into the culture medium. Cells were further cultured for 24 or 48 h based on experimental purposes. For inhibition of autophagy, HL‐60 cells were treated with 200 nM bafilomycin A1 or 10 µM chloroquine. Overexpression and knockdown were performed by transfection. (Data sourced from selleck products)
Animal Study:^{^[6]}	Animal Models Female NOD/SCID athymic mice injected with MDAMB231 cells Dosages 30 mg/kg Administration o.g. (Data sourced from selleck products)

Cell Assay:^{^[5]}

Cell lines
HL‐60 cells
Concentrations
10 µM
Incubation Time
48 h
Method
One day before treatment, AML cells were seeded with a density of 2 × 10⁵ cells/mL. Fresh medium containing different concentrations of Ara‐C was added into the culture medium. Cells were further cultured for 24 or 48 h based on experimental purposes. For inhibition of autophagy, HL‐60 cells were treated with 200 nM bafilomycin A1 or 10 µM chloroquine. Overexpression and knockdown were performed by transfection.

(Data sourced from selleck products)

Animal Study:^{^[6]}

Animal Models
Female NOD/SCID athymic mice injected with MDAMB231 cells
Dosages
30 mg/kg
Administration
o.g.

(Data sourced from selleck products)

References

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Selleck's Chloroquine has been cited by 158 publications

Tet methylcytosine dioxygenase 2 (TET2) deficiency elicits EGFR-TKI (tyrosine kinase inhibitors) resistance in non-small cell lung cancer [ Signal Transduct Target Ther, 2024, 9(1):65]	PubMed: 38461173
Engineered a dual-targeting HA-TPP/A nanoparticle for combination therapy against KRAS-TP53 co-mutation in gastrointestinal cancers [ Bioact Mater, 2024, 32:277-291]	PubMed: 37876556
Autophagy regulates the maturation of hematopoietic precursors in the embryo [ Nat Commun, 2024, 15(1):2255]	PubMed: 38490977
Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies [ Cancer Commun (Lond), 2024, 44(3):408-432]	PubMed: 38407943
Dysregulated CREB3 cleavage at the nuclear membrane induces karyoptosis-mediated cell death [ Exp Mol Med, 2024, 56(3):686-699]	PubMed: 38480902
Fut2 Deficiency Promotes Intestinal Stem Cell Aging by Damaging Mitochondrial Functions via Down-Regulating α1,2-Fucosylation of Asah2 and Npc1 [ Research (Wash D C), 2024, 7:0343]	PubMed: 38550777
Sonic hedgehog restrains the ubiquitin-dependent degradation of SP1 to inhibit neuronal/glial senescence associated phenotypes in chemotherapy-induced peripheral neuropathy via the TRIM25-CXCL13 axis [ J Adv Res, 2024, S2090-1232(24)00106-1]	PubMed: 38479571
Inhibiting autophagy enhanced mitotic catastrophe-mediated anticancer immune responses by regulating the cGAS-STING pathway [ Cancer Lett, 2024, 586:216695]	PubMed: 38325769
Pinacidil ameliorates cardiac microvascular ischemia-reperfusion injury by inhibiting chaperone-mediated autophagy of calreticulin [ Basic Res Cardiol, 2024, 119(1):113-131]	PubMed: 38168863
The DNA damage-independent ATM signalling maintains CBP/DOT1L axis in MLL rearranged acute myeloid leukaemia [ Oncogene, 2024, 10.1038/s41388-024-02998-2]	PubMed: 38671157

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SHIPPING AND STORAGE
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