CDN1163

Catalog No.S6815 Batch:S681502

Technical Data

Formula	C₂₀H₂₀N₂O₂
Molecular Weight	320.39	CAS No.	892711-75-0
Solubility (25°C)*	In vitro	DMSO	64 mg/mL (199.75 mM)
		Ethanol	64 mg/mL (199.75 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

CDN1163 is a small molecular allosteric activator of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) that improves Ca2+ homeostasis.

Targets

SERCA ^[1]

In vitro

CDN1163 treatment significantly reduces the hepatic expression of genes involved in gluconeogenesis and lipogenesis, attenuates ER stress response and ER stress-induced apoptosis, and improves mitochondrial biogenesis, possibly through SERCA2-mediated activation of AMP-activated protein kinase pathway.^[1]

In vivo

CDN1163 reduces blood glucose levels and improves metabolic parameters and gluconeogenic gene expression, reverses hepatic steatosis, inhibits ER stress and ER stress-induced apoptosis, and improves mitochondrial efficiency in ob/ob mice in vivo.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HEK cells Concentrations 10 μm Incubation Time 2 h Method Cells are grown in 96-well plates (n = 6) and exposed to CDN1163 (10 μm) or vehicle (DMSO) for 2 h followed by the addition of 200 μm H²O² compared with untreated controls. H²O² activates the unfolded protein response and is an inducer of ER stress-promoted apoptosis. After 16 h of incubation, cell viability is measured using Luminescent Cell Viability Assay.

Cell Assay:^{^[1]}

Cell lines
HEK cells
Concentrations
10 μm
Incubation Time
2 h
Method
Cells are grown in 96-well plates (n = 6) and exposed to CDN1163 (10 μm) or vehicle (DMSO) for 2 h followed by the addition of 200 μm H²O² compared with untreated controls. H²O² activates the unfolded protein response and is an inducer of ER stress-promoted apoptosis. After 16 h of incubation, cell viability is measured using Luminescent Cell Viability Assay.

References

[1] Kang S, et al. J Biol Chem. 2016 Mar 4;291(10):5185-98.

Selleck's CDN1163 has been cited by 2 publications

Aberrant miR-339-5p/neuronatin signaling causes prodromal neuronal calcium dyshomeostasis in mutant presenilin mice [ J Clin Invest, 2022, 132(8)e149160]	PubMed: 35426376
Restoration of Sarco/Endoplasmic Reticulum Ca2+-ATPase Activity Functions as a Pivotal Therapeutic Target of Anti-Glutamate-Induced Excitotoxicity to Attenuate Endoplasmic Reticulum Ca2+ Depletion [ Front Pharmacol, 2022, 13:877175]	PubMed: 35517826

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SHIPPING AND STORAGE
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