Carnosic acid

Catalog No.S3838 Batch:S383801

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Technical Data

Formula

C20H28O4
Molecular Weight 332.43 CAS No. 3650-09-7
Solubility (25°C)* In vitro DMSO 66 mg/mL (198.53 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Carnosic acid (Salvin) is a phenolic diterpene, endowed with antioxidative and antimicrobial properties. It is increasingly used within food, nutritional health and cosmetics industries.
In vitro Carnosic acid (CA) would inhibit the cell viability of three CRC cell lines: Caco-2, HT29 and LoVo in a dose-dependent manner, with IC50 values in the range of 24-96 μM. CA induces cell death by apoptosis in Caco-2 line after 24 h of treatment and inhibits cell adhesion and migration, possibly by reducing the activity of secreted proteases such as urokinase plasminogen activator (uPA) and metalloproteinases (MMPs). CA downregulates the expression of COX-2 in Caco-2 cells at both the mRNA and protein levels[1]. CA induces antioxidant and anti-inflammatory effects in several cell types, as observed in both in vitro and in vivo experimental models. It exhibits the ability to promote mitochondrial protection in neural cells[2]. In vitro, CA suppresses Receptor Activator for Nuclear Factor-κ B Ligand (RANKL)- and Macrophage colony-stimulating factor (M-CSF)-induced osteoclastogenesis. The osteoclastic specific markers are inhibited by CA. Signal transduction studies show that CA significantly decreases the expression of molecules contributing to ROS and increases anti-oxidants. Additionally, CA inactivates the RANKL- and M-CSF-induced p38 mitogen activated protein kinases (MAPK), inhibits NF-κB phosphorylation, causing pro-inflammatory cytokines down-regulation. CA ameliorates acute promyelocytic leukemia cells through activating nuclear transcription factor E2-related factor 2 (Nrf2), which plays an essential role in cytoprotective responses to oxidative stress[3].
In vivo CA significantly down-regulates fasting blood glucose, glucose level in oral glucose tolerance test (OGTT) and insulin tolerance test (ITT), ameliorates CIA-induced bone loss, and reduces pro-inflammatory cytokines and reactive oxygen species (ROS) in db/db mice with arthritis induced by collagen-induced arthritis (CIA). CA could regulate ER stress to ameliorate disease progression. It can also cross the blood-brain barrier readily and exert its protective effects via its potent anti-oxidative role[3].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    Human colon carcinoma cell lines, Caco-2, HT29 and LoVo

  • Concentrations

    0 to 388 μM

  • Incubation Time

    24 h

  • Method

    Cells (1×104) are seeded in 96-well microplates in complete medium. After 48 h, cells are washed twice with PBS and treated with RE, RA and CA (concentration range from 0 to 388 μM) in complete medium for 24 h. Cell viability is assessed.

Animal Study:[3]
  • Animal Models

    male C57BL/KsJ-db/db (db/db) mice

  • Dosages

    30 mg/kg and 60 mg/kg

  • Administration

    i.p.

Selleck's Carnosic acid has been cited by 3 publications

Pilot-Scale Antioxidant Dipping of Herring (Clupea harengus) Co-products to Allow Their Upgrading to a High-Quality Mince for Food Production [ ACS Sustain Chem Eng, 2023, 11(12):4727-4737] PubMed: 37013165
NQO1 Is a Determinant for Cellular Sensitivity to Anti-Tumor Agent Napabucasin [ Am J Cancer Res, 2020, 1;10(5):1442-1454] PubMed: 32509390
Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species [ J Reprod Dev, 2020, 10.1262/jrd.2020-086] PubMed: 33055461

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.