Borneol

Catalog No.S3605 Batch:S360501

Print

Technical Data

Formula

C10H18O
Molecular Weight 154.25 CAS No. 507-70-0
Solubility (25°C)* In vitro DMSO 30 mg/mL (194.48 mM)
Ethanol 30 mg/mL (194.48 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Borneol is a bicyclic monoterpenoid compound extracted from medicinal plants such as Blumea balsamifera, and Dryobalanops aromatica. It is a TRPM8 agonist that increases ocular surface wetness.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
Targets
TRPM8 [2]
(cell-based assay)
65 μM(EC50)
In vitro Borneol activates TRPM8 channel in a temperature- and dose-dependent manner. Borneol at micromolar concentrations do not affect the viability of human corneal epithelial cells. Borneol has been found to activate TRPV3 channel, which belongs to the heat-activated vanilloid family of TRP channels[1]. Borneol can potentiate (EC50 = 248 μM) or directly activate (> 1.5 mM) the GABAA receptor, activate the TRPV3 channel (EC50 = 3.45 ± 0.13 mM), and inhibit the TRPA1 channel (IC50 = 0.5 ± 0.3 or 0.20 ± 0.06 mM)[2].
In vivo Borneol is widely used in ophthalmic preparations and classic formulas of Traditional Chinese Medicine to treat various diseases including oculopathies, oral ulcers, sore throat, skin diseases and mild neurological disorders, and found to produce anti-inflammatory, anti-bacterial and analgesic effects. Borneol can increase tear secretion via activating the cold-sensing TRPM8 channel in the cornea[1]. borneol may facilitate the delivery of other effective components and enhance their effects in combinatorial herbal formulas. Thus, borneol is also orally administered as an adjuvant component for the treatment of a variety of diseases. topical borneol provides analgesic effects in three different mouse models of pain[2].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HEK293 and HCE cells

  • Concentrations

    --

  • Incubation Time

    24 h

  • Method

    The viability of HEK293 and HCE cells is measured with WST-1 reagent. Briefly, cells are seeded in 96-well plates to reach a confluency of 50–60%. Borneol or menthol is added into the cell culture medium and incubated for 24 h. WST-1 (10 μl) is then added into each well and incubated for 2 h. Absorbance at 450 nm (A450) and 690 nm (A690) is measured in a plate reader. The subtracted value (A450-A690) is used to evaluate viability of the cells.

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.