β-Glycerophosphate sodium salt hydrate

Catalog No.S3620 Batch:S362001

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Technical Data

Formula

C3H9O6P.5H2O.2Na
Molecular Weight 306.11 CAS No. 13408-09-8
Solubility (25°C)* In vitro Water 61 mg/mL (199.27 mM)
DMSO Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description β-Glycerophosphate (sodium salt hydrate) is a potent protein phosphatase inhibitor.
Targets
phosphatase [1]
In vitro β-glycerophosphate accelerates in vitro calcification by BVSMC and induces extensive calcium deposition in a manner analogous to in vitro mineralization by osteoblasts. β-glycerophosphate can increase ALP activity in osteoblasts[2].

Protocol (from reference)

Cell Assay:[2]
  • Cell lines

    BVSMC (bovine vascular smooth muscle cells)

  • Concentrations

    10 mmol/L

  • Incubation Time

    14 d

  • Method

    BVSMC are cultured in DMEM containing 10 mmol/L sodium pyruvate supplemented with 15% FBS (growing medium). After confluence, the cells are inoculated in DMEM containing 10 mmol/L sodium pyruvate supplemented with 15% FBS in the presence of 10 mmol/L β-glycerophosphate, 10-7 mol/L insulin, and 50 μg/mL of ascorbic acid for 14 days (calcification medium). The medium is replaced with fresh medium every 3 days. In certain experiments, only β-glycerophosphate is used to induce BVSMC calcification. Moreover, in the time-course experiments, before being cultured in calcification medium (defined as day 0), cells are selected to be used as controls.

Selleck's β-Glycerophosphate sodium salt hydrate has been cited by 9 publications

Long noncoding RNA Malat1 inhibits Tead3-Nfatc1–mediated osteoclastogenesis to suppress osteoporosis and bone metastasis [ Res Sq, 2023, rs.3.rs-2405644] PubMed: 36993303
Long noncoding RNA Malat1 inhibits Tead3-Nfatc1-mediated osteoclastogenesis to suppress osteoporosis and bone metastasis [ Res Sq, 2023, rs.3.rs-2405644] PubMed: 36993303
Sphingosine kinase 1 promotes tumor immune evasion by regulating the MTA3-PD-L1 axis [ Cell Mol Immunol, 2022, 19(10):1153-1167] PubMed: 36050478
Establishment and characterization of immortalized sweat gland myoepithelial cells [ Sci Rep, 2022, 12(1):7] PubMed: 34997030
A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma [ Mol Med Rep, 2022, 26(2)255] PubMed: 35703357
MiR-140-5p upregulation suppressed β-glycerophosphate-induced vascular smooth muscle cell calcification via targeting TLR4 [ Immunopharmacol Immunotoxicol, 2022, 1-11] PubMed: 35272550
Identification of a Three-Glycolysis-Related lncRNA Signature Correlated With Prognosis and Metastasis in Clear Cell Renal Cell Carcinoma [ Front Med (Lausanne), 2021, 8:777507] PubMed: 35083240
ADORA1 Inhibition Promotes Tumor Immune Evasion by Regulating the ATF3-PD-L1 Axis [ Cancer Cell, 2020, 37(3):324-339.e8] PubMed: 32183950
ADORA1 Inhibition Promotes Tumor Immune Evasion by Regulating the ATF3-PD-L1 Axis. [ Cancer Cell, 2020, 37(3):324-339] PubMed: 32183950

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.