Benzamide

Catalog No.S4715 Batch:S471501

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Technical Data

Formula

C7H7NO
Molecular Weight 121.14 CAS No. 55-21-0
Solubility (25°C)* In vitro DMSO 24 mg/mL (198.11 mM)
Ethanol 24 mg/mL (198.11 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Benzamide, a derivative of benzoic acid, is an inhibitor of poly(ADP-ribose) polymerase with an IC50 of 3.3 μM.
Targets
PARP [4]
(Cell-free assay)
3.3 μM
In vitro Benzamide, an inhibitor of PARP is protective against excitatory amino acid-induced cell death in primary cultures of neurons derived from neonatal rat brain. In addition, benzamide has more recently been shown to partially prevent the loss of dopaminergic cells and the increase in reactive gliosis caused by METH in vitro in fetal rat mesencephalic cells in culture[1]. Benzamide prevents transformation in a cell cycle-specific manner, maximal prevention coinciding with early S phase, also characteristic of maximal susceptibility to transformation[2].
In vivo PARP inhibitor benzamide is neuroprotective in C57Bl/6N mice against different types of neurotoxicities and without affecting body temperature[2]. Benzamide treatment significantly decreases the iNOS expression and number of apoptotic neurons and thereby improves the neuronal survival and memory during GCI. Benzamide administration (160 mg/kg i.p.) does not induce hypothermia and reaches the CNS in 30 min in the concentration range of 0.09-0.64 mM, at which, it shows neuroprotection[3].

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Primary human fibroblasts

  • Concentrations

    1 mM

  • Incubation Time

    10 hr

  • Method

    Exposure to carcinogens and benzamide is done 10 hr after the release of the metabolically induced G1/S block and exposure lasts 10 hr, followed by three washings and refeeding with fresh media.

Selleck's Benzamide has been cited by 3 publications

Unravelling the Regions of Mutant F508del-CFTR More Susceptible to the Action of Four Cystic Fibrosis Correctors. [ Int J Mol Sci, 2019, 20(21)] PubMed: 31683989
CDK5 Inhibitor Downregulates Mcl-1 and Sensitizes Pancreatic Cancer Cell Lines to Navitoclax [ Mol Pharmacol, 2019, 96(4):419-429] PubMed: 31467029
Overcoming multiple drug resistance mechanisms in medulloblastoma. [Othman RT, et al. Acta Neuropathol Commun, 2014, 2:57] PubMed: 24887326

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.