(+)-α-Lipoic acid

Catalog No.S3998 Batch:S399801

Technical Data

Formula	C₈H₁₄O₂S₂
Molecular Weight	206.33	CAS No.	1200-22-2
Solubility (25°C)*	In vitro	DMSO	41 mg/mL (198.71 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

(+)-α-Lipoic acid ((R)-(+)-α-Lipoic acid, α-Lipoic acid, Alpha-Lipoic acid), a physiological form of thioctic acid, is a strong antioxidant that relieves diabetic neuropathic symptoms. It shows superior antioxidative effects to its racemate. R(+)-α-lipoic acid is an essential cofactor of mitochondrial enzyme complexes. R(+)-α-lipoic acid inhibits NF-κB-dependent HIV-1 LTR activation.

Targets

NF-κB ^[2]

LTR ^[1]

LTR ^[2]

In vitro

Using L6 myotubes and 3T3-L1 adipocytes as a model of muscle and fat cells in culture, R(+)-α-lipoic acid (R-LA) is shown to increase tyrosine phosphorylation of insulin receptor and IRS-1, to enhance PI 3-kinase and Akt1 activities, to elevate GLUT4 content in the plasma membranes, and to increase glucose uptake into the cells. 3T3-L1 adipocytes possess a low capacity to reduce R-α-lipoic acid and the oxidized isoforms are effective in stimulating glucose transport. R-α-lipoic acid modulates glucose transport by changing intracellular redox status. Insulin receptor is a target of R-LA action^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines 3T3-L1 preadipocytes Concentrations 250 μM Incubation Time 2-48 h Method Cells seeded in 12-well plates were incubated for 2–48 h with R-LA (250 μM) and loaded with H2DCF-DA (20 μM) for the last 30 min in PBS containing R-LA. For some experiments cells were washed and incubated with R-LA (0.25-1 μM) and H2DCF-DA (20 μM) for 30 min. Following the treatments, cells were gently scraped by a lifter and suspended in the same media. For detection of intracellular fluorescence, cells were excited using a 488-nm argon-ion laser in a flow cytometer. The dichlorofluorescein (DCF) emission was recorded at 530 nm. Data were collected from at least 20,000 cells.

Cell Assay:^{^[1]}

Cell lines
3T3-L1 preadipocytes
Concentrations
250 μM
Incubation Time
2-48 h
Method
Cells seeded in 12-well plates were incubated for 2–48 h with R-LA (250 μM) and loaded with H2DCF-DA (20 μM) for the last 30 min in PBS containing R-LA. For some experiments cells were washed and incubated with R-LA (0.25-1 μM) and H2DCF-DA (20 μM) for 30 min. Following the treatments, cells were gently scraped by a lifter and suspended in the same media. For detection of intracellular fluorescence, cells were excited using a 488-nm argon-ion laser in a flow cytometer. The dichlorofluorescein (DCF) emission was recorded at 530 nm. Data were collected from at least 20,000 cells.

References

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SHIPPING AND STORAGE
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