Allicin

Catalog No.S3860 Batch:S386002

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Technical Data

Formula

C6H10OS2

Molecular Weight 162.27 CAS No. 539-86-6
Solubility (25°C)* In vitro
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description Allicin (Diallyl Thiosulfinate), the main biologically active component of the freshly crushed garlic extracts, possesses various biological activities including antibacterial, antifungal and antiparasitic effects., Allicin (Diallyl Thiosulfinate), the main biologically active component of the freshly crushed garlic extracts, possesses various biological activities including antibacterial, antifungal and antiparasitic effects.
In vitro Allicin is readily permeable through phospholipid membranes aiding its biological activity. It inhibits the growth of cancer cells of murine and human origin. Allicin induces the formation of apoptotic bodies, nuclear condensation and a typical DNA ladder in cancer cells. Furthermore, activation of caspases-3, -8 and -9 and cleavage of poly(ADP-ribose) polymerase are induced by allicin[1]. Allicin is highly reactive with thiol-groups but under certain conditions it also reacts with itself forming further compounds which can also be bioactive, such as vinyl-dithiins, ajoene and polysulfanes. Since allicin is able to oxidize biomolecules such as glutathione or protein cysteine residues under physiological conditions, it fulfils the definition of a Reactive Sulfur Species (RSS). Allicin certainly acts as a “physiological antioxidant” by inducing the cellular “phase II detoxification system”. Allicin activates the redox-dependent mammalian transcription factor Nrf-2 the localization of which depends upon the Keap1 protein (Kelch-like ECH1-associated protein), presumably by oxidizing the regulatory cysteine-residues, as demonstrated for the YAP1-transcription factor which is a functional homologue of the Nrf-2 system in yeast[1]. In vitro studies show that allicin inhibits TNF-α-mediated T cell adhesion to extracellular matrix components and to endothelial cells. Allicin also inhibits TNF-α-mediated intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression on human vascular endothelial cells.
In vivo Allicin has a protective effect on immune-mediated, Con A-induced hepatitis in mice[2].
Density 1.109 g/mL

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    L-929, SW480 and HeLa cells

  • Concentrations

    0-100 μM

  • Incubation Time

    24 or 48 h

  • Method

    Cell viability assays are carried out as described with slight modifications. Briefly, cells are seeded at a density of 3×104 cells/well into 24-well plates. After 24 h, allicin is added to the medium at various concentrations and incubated for 24 or 48 h as indicated. At the end of the incubation, 50 μl of MTT (2 mg/ml) per well is added, and the formazan crystals formed are solubilized in acidified isopropanol after aspirating the medium. The extent of MTT reduction is measured spectrophotometrically at 570 nm, and the cell survival is expressed as percentage over the untreated control.

Animal Study:[2]
  • Animal Models

    Balb/c male mice

  • Dosages

    8-20 mg/kg/day

  • Administration

    by oral gavage

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.