Adalimumab (anti-TNF-alpha)

Catalog No.A2010        Batch: A201003

Print

Technical Data

CAS No. 331731-18-1
Formulation PBS buffer, pH 7.2
Isotype Human IgG1
Source CHO cells
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 99.23%
Protein concentration 5.00 mg/ml
Endotoxin Level ≤1 EU/mg

Biological Activity

Description Adalimumab (anti-TNF-alpha) is the first fully human, recombinant IgG1 monoclonal antibody that specifically targets human TNF-alpha, MW: 144.19 KD.
Targets
TNF-α [1]
In vitro

Adalimumab treatment alters the secretion of cytokines.[1]
In vivo

We injected adalimumab intraductally into the MG of adult mice;this was found to stimulate BSC multipotency.[2]

Protocol (Only for Reference)

Cell Assay:

  • Fa2N-4 cells were co-cultivated (CC) with monocyte derived macrophages (MDMs) for 24 h. Physiological (15%) and pro-inflammatory (50%) MDM-to-Fa2N-4 ratios were used. All measured cytokine concentrations were related to the corresponding control (fold change) to analyse (A) the IL-8 neutralising antibody (αIL-8, 100 ng/ml) specific effect and (B) the TNF-α neutralising effect of Adalimumab.
Animal Study:

  • Animal Models: K5CreER, K8rtTA, TetO-DTA mice
    Dosages: 0.15 mg
    Administration: i.d.
    Reference:[2]

Selleck's Adalimumab (anti-TNF-alpha) has been cited by 12 publications

Autoimmunity in Down's syndrome via cytokines, CD4 T cells and CD11c+ B cells [ Nature, 2023, 615(7951):305-314] PubMed: 36813963
HS 3D-SeboSkin Model Enables the Preclinical Exploration of Therapeutic Candidates for Hidradenitis Suppurativa/Acne Inversa [ Pharmaceutics, 2023, 15(2)619] PubMed: 36839941
Identification of hub necroptosis-related lncRNAs for prognosis prediction of esophageal carcinoma [ Aging (Albany NY), 2023, 15(11):4794-4819] PubMed: 37263709
Characterization and optimization of variability in a human colonic epithelium culture model [ bioRxiv, 2023, 2023.09.22.559007] PubMed: 37790345
Characterization and optimization of variability in a human colonic epithelium culture model [ bioRxiv, 2023, 2023.09.22.559007] PubMed: 37790345
Tumor necrosis factor alpha delivers exogenous inflammation-related microRNAs to recipient cells with functional targeting capabilities [ Mol Ther, 2022, S1525-0016(22)00418-X] PubMed: 35791880
Novel indirect co-culture of immortalised hepatocytes with monocyte derived macrophages is characterised by pro-inflammatory cytokine networks [ Toxicol In Vitro, 2021, 73:105134] PubMed: 33662514
Protective Effect of Adalimumab on Diabetic Nephropathy by Regulating TNF-α Signal Pathway [ Iran J Public Health, 2021, 50(12):2536-2545] PubMed: 36317034
Heterotypic cell-cell communication regulates glandular stem cell multipotency [ Nature, 2020, 584(7822):608-613] PubMed: 32848220
Heterotypic cell-cell communication regulates glandular stem cell multipotency [ Nature, 2020, 584(7822):608-613] PubMed: 32848220

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

Specific storage and handling information for each product is indicated on the product datasheet. Most Selleck products are stable under the recommended conditions. Products are sometimes shipped at a temperature that differs from the recommended storage temperature. Short-term storage of many products are stable in the short-term at temperatures that differ from that required for long-term storage.
We ensure that the product is shipped under conditions that will maintain the quality of the reagents. Upon receipt of the product, follow the storage recommendations on the product data sheet.