Fisetin

Catalog No.S2298 Batch:S229809

Technical Data

Formula	C₁₅H₁₀O₆
Molecular Weight	286.24	CAS No.	528-48-3
Solubility (25°C)*	In vitro	DMSO	57 mg/mL (199.13 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Fisetin (Fustel) is a potent sirtuin activating compound (STAC) and an agent that modulates sirtuins.

Targets

Sirtuin ^[1]

In vitro

Fisetin selectively kills SPiDER-β-Gal positive senescence neural cells in vitro.^[2]

In vivo

Oral administration of fisetin, a senolytic drug, reduces the number of senescent neural cells observed, the SASP expression level, and depressive behavior in MRL/lpr mice.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells Concentrations 5, 10, or 20 μM Incubation Time 48 h Method Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells are seeded on a 96-well black/clear bottom plate at 40,000 cells and 10,000 cells per well, respectively. After senescence induction for 6 days, 5 μM, 10 μM, or 20 μM fisetin is added and the cells are incubated for 48 h. Cell number and cellular senescence are determined by DAPI staining and SPiDER-β-Gal staining, respectively. Cells are washed twice with PBS, fixed in 4% paraformaldehyde at room temperature for 5 min, and washed twice with PBS. Sections are incubated in 20 M SPiDER-β-gal in solution in McIlvaine buffer (pH 6.0) for 60 min at 37 ℃. After washing of tissue sections, nuclei are stained with DAPI. Cells are observed.
Animal Study:^{^[2]}	Animal Models Female MRL/lpr mice, haplotype-matched female MRL/MPJ mice Dosages 100 mg/kg Administration p.o.

Cell Assay:^{^[2]}

Cell lines
Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells
Concentrations
5, 10, or 20 μM
Incubation Time
48 h
Method
Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells are seeded on a 96-well black/clear bottom plate at 40,000 cells and 10,000 cells per well, respectively. After senescence induction for 6 days, 5 μM, 10 μM, or 20 μM fisetin is added and the cells are incubated for 48 h. Cell number and cellular senescence are determined by DAPI staining and SPiDER-β-Gal staining, respectively. Cells are washed twice with PBS, fixed in 4% paraformaldehyde at room temperature for 5 min, and washed twice with PBS. Sections are incubated in 20 M SPiDER-β-gal in solution in McIlvaine buffer (pH 6.0) for 60 min at 37 ℃. After washing of tissue sections, nuclei are stained with DAPI. Cells are observed.

Animal Study:^{^[2]}

Animal Models
Female MRL/lpr mice, haplotype-matched female MRL/MPJ mice
Dosages
100 mg/kg
Administration
p.o.

References

Customer Product Validation

: Data from [Data independently produced by , , Alcohol Clin Exp Res, 2016, 40(10):2076-2084.]

Selleck's Fisetin has been cited by 35 publications

Clearance of defective muscle stem cells by senolytics restores myogenesis in myotonic dystrophy type 1 [ Nat Commun, 2023, 14(1):4033]	PubMed: 37468473
Fisetin ameliorates fibrotic kidney disease in mice via inhibiting ACSL4-mediated tubular ferroptosis [ Acta Pharmacol Sin, 2023, 10.1038/s41401-023-01156-w]	PubMed: 37696989
Tubular cell senescence promotes maladaptive kidney repair and chronic kidney disease after cisplatin nephrotoxicity [ JCI Insight, 2023, 8(8)e166643]	PubMed: 36917180
Post-translational modification of CDK1-STAT3 signaling by fisetin suppresses pancreatic cancer stem cell properties [ Cell Biosci, 2023, 13(1):176]	PubMed: 37743465
PPARγ activation by fisetin mitigates vascular smooth muscle cell senescence via the mTORC2-FoxO3a-autophagy signaling pathway [ Biochem Pharmacol, 2023, S0006-2952(23)00485-9]	PubMed: 37890594
Sexual dimorphic metabolic and cognitive responses of C57BL/6 mice to Fisetin or Dasatinib and quercetin cocktail oral treatment [ Geroscience, 2023, 45(5):2835-2850]	PubMed: 37296266
Fisetin, a Natural Polyphenol, Ameliorates Endometriosis Modulating Mast Cells Derived NLRP-3 Inflammasome Pathway and Oxidative Stress [ Int J Mol Sci, 2023, 24(6)5076]	PubMed: 36982152
Fisetin Attenuates Cellular Senescence Accumulation During Culture Expansion of Human Adipose-Derived Stem Cells [ Stem Cells, 2023, 41(7):698-710]	PubMed: 37279940
Fisetin alleviates cellular senescence through PTEN mediated inhibition of PKCδ-NOX1 pathway in vascular smooth muscle cells [ Arch Gerontol Geriatr, 2023, 108:104927]	PubMed: 36645971
Network pharmacology identifies fisetin as a treatment for osteoporosis that activates the Wnt/β-catenin signaling pathway in BMSCs [ J Orthop Surg Res, 2023, 18(1):312]	PubMed: 37087476

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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