Dexamethasone

Catalog No.S1322 Batch:S132205

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Technical Data

Formula

C22H29FO5
Molecular Weight 392.46 CAS No. 50-02-2
Solubility (25°C)* In vitro DMSO 78 mg/mL (198.74 mM)
Ethanol 20 mg/mL (50.96 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO Corn oil
1.35mg/ml Taking the 1 mL working solution as an example, add 50 μL of 27 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Dexamethasone is a potent synthetic member of the glucocorticoid class of steroid drugs, and an interleukin receptor modulator that has anti-inflammatory and immunosuppressant effects. Dexamethasone induces autophagy and mitophagy. Dexamethasone is tested in hospitalized patients with COVID-19 and is found to have benefits for critically ill patients.
Targets
interleukin receptor [1]
(Cell-free assay)
In vitro

Dexamethasone results in decrease in transmonolayer paracellular permeability mainly to sucrose, fluorescein and dextrans of up to 20 KDa in an immortalised rat brain endothelial cell line (GPNT). Dexamethasone results in filamentous actin and the cytoskeleton associated protein cortactin being highly concentrated in the regions of cell-cell contact with few F-actin stress fibres visible within the cytoplasm in cultured rat brain endothelial cells, an observation consistent with a more differentiated barrier phenotype induced by dexamethasone. Dexamethasone treatment has been shown to strongly stimulate the level of the Id-1 protein, which is a serum-inducible helix-loop-helix transcriptional repressor, involved in cell differentiation, and this effect was shown to be associated with reorganisation of ZO-1 to the cell periphery in Con8 mammary epithelial tumor cells. Dexamethasone prevents cytokine-induced enhanced expression of MMP-9 and alterations in the expression of ZO-1 in untreated GPNT monolayers. [1]

Dexamethasone depletes both basal and TNF-alpha-stimulated GSH levels by down-regulating the gamma-GCS-heavy subunit transcription via a mechanism involving AP-1 (c-Jun) in alveolar epithelial cells. Dexamethasone decreases both basal and stimulated GSH levels (TNF-α-treated) in alveolar epithelial cells (A549), without any change in GSSG. [2]
In vivo

Dexamethasone is administered i.m. to pregnant ewes, leads to the following results (1) blood pressure is unchanged; (2) as previously reported in the fetus, sensitivity to endothelin-1 (ET) is increased; (3) acetylcholine-induced relaxation is increased; (4) L-NAME suppressible vasodilatory response to ET is abolished; (5) there is no change in endothelium-independent vasodilatation; and (6) there is no change in eNOS RNA and protein levels, when compared to saline treated controls. [3]

Protocol (from reference)

Cell Assay:

[6]
  • Cell lines

    CB CD34+ cells

  • Concentrations

    1 μM

  • Incubation Time

    16 h

  • Method

    Cells were treated with indicated concentrations of drug.
Animal Study:

[7]
  • Animal Models

    Mice

  • Dosages

    1 mg/kg

  • Administration

    i.p.

Customer Product Validation

Data from [Oncogene, 2013, 32, 1316-29]

Data from [Oncogene, 2013, 32, 1316-29]

Data from [Data independently produced by , , Int J Oncol, 2018, doi:10.3892/ijo.2018.4635]

Selleck's Dexamethasone has been cited by 106 publications

Virological characteristics of the SARS-CoV-2 BA.2.86 variant [ Cell Host Microbe, 2024, S1931-3128(24)00005-2] PubMed: 38280382
Virological characteristics of the SARS-CoV-2 Omicron XBB.1.5 variant [ Nat Commun, 2024, 15(1):1176] PubMed: 38332154
A novel strategy to generate immunocytokines with activity-on-demand using small molecule inhibitors [ EMBO Mol Med, 2024, 16(4):904-926] PubMed: 38448543
Glucocorticoid treatment influences prostate cancer cell growth and the tumor microenvironment via altered glucocorticoid receptor signaling in prostate fibroblasts [ Oncogene, 2024, 43(4):235-247] PubMed: 38017134
β-catenin inhibition disrupts the homeostasis of osteogenic/adipogenic differentiation leading to the development of glucocorticoid-induced osteonecrosis of the femoral head [ Elife, 2024, 12RP92469] PubMed: 38376133
Inhibition of PTPRE suppresses tumor progression and improves sorafenib response in hepatocellular carcinoma [ Biomed Pharmacother, 2024, 173:116366] PubMed: 38458013
Kupffer cells abrogate homing and repopulation of allogeneic hepatic progenitors in injured liver site [ Stem Cell Res Ther, 2024, 15(1):48] PubMed: 38378583
Serine to proline mutation at position 341 of MYOC impairs trabecular meshwork function by causing autophagy deregulation [ Cell Death Discov, 2024, 10(1):21] PubMed: 38212635
The ESCRT protein CHMP5 promotes T cell leukemia by controlling BRD4-p300-dependent transcription [ bioRxiv, 2024, 2024.01.29.577409] PubMed: 38352301
LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia [ J Hematol Oncol, 2023, 16(1):30] PubMed: 36973755

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.