8-Bromo-cAMP

Catalog No.S7857 Batch:S785707

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Technical Data

Formula

C10H10BrN5NaO6P
Molecular Weight 430.08 CAS No. 76939-46-3
Solubility (25°C)* In vitro DMSO 125 mg/mL (290.64 mM)
Water 100 mg/mL (232.51 mM)
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
4.3mg/ml Taking the 1 mL working solution as an example, add 50 μL 86 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description 8-bromo-cAMP (8-Br-Camp) is a cell permeable analog of cAMP that activates cyclic-AMP-dependent protein kinase with a Ka value of 0.05 μM; and a PKA activator.
Targets
PKA [1]
0.05 μM(Ka)
In vitro

8-Bromo-cAMP improves the reprogramming efficiency of human neonatal foreskin fibroblast (HFF1) cells. [1]

In bronchial epithelial cells, 8-Bromo-cAMP decreases HDE-stimulated tumor necrosis factor (TNF)-α-converting enzyme (TACE; ADAM-17) activity and subsequent TNF-α release. [2]

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    BEAS-2B cells

  • Concentrations

    100 μM

  • Incubation Time

    1 h

  • Method

    Cells were treated with various concentrations of 8-Br-cAMP.
Animal Study:

[3]
  • Animal Models

    Sprague-Dawley rats

  • Dosages

    5 mg/kg

  • Administration

    i.c.v.

Customer Product Validation

, , Development, 2016, 143(12):2103-10.

Data from [Data independently produced by , , J Cell Mol Med, 2018, 22(11):5518-5532]

Selleck's 8-Bromo-cAMP has been cited by 25 publications

β3-Adrenoceptor as a new player in the sympathetic regulation of the renal acid-base homeostasis [ Front Physiol, 2024, 15:1304375] PubMed: 38455846
Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm [ Protein Cell, 2023, 14(4):262-278] PubMed: 37084236
METTL3-regulated m6A modification impairs the decidualization of endometrial stromal cells by regulating YTHDF2-mediated degradation of FOXO1 mRNA in endometriosis-related infertility [ Reprod Biol Endocrinol, 2023, 21(1):99] PubMed: 37891533
METTL3-regulated m6A modification impairs the decidualization of endometrial stromal cells by regulating YTHDF2-mediated degradation of FOXO1 mRNA in endometriosis-related infertility [ Reprod Biol Endocrinol, 2023, 21(1):99] PubMed: 37891533
High extracellular glucose promotes cell motility by modulating cell deformability and contractility via the cAMP-RhoA-ROCK axis in human breast cancer cells [ Mol Biol Cell, 2023, 34(8):ar79] PubMed: 37195739
Derivation of totipotent-like stem cells with blastocyst-like structure forming potential [ Cell Res, 2022, 10.1038/s41422-022-00668-0] PubMed: 35508506
Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma [ Signal Transduct Target Ther, 2022, 7(1):46] PubMed: 35169126
Elimination of Reprogramming Transgenes Facilitates the Differentiation of Induced Pluripotent Stem Cells into Hepatocyte-like Cells and Hepatic Organoids [ Biology (Basel), 2022, 11(4)493] PubMed: 35453693
Inhibition of PDE10A-Rescued TBI-Induced Neuroinflammation and Apoptosis through the cAMP/PKA/NLRP3 Pathway [ Evid Based Complement Alternat Med, 2022, 2022:3311250] PubMed: 35463083
Protein modifications throughout the lung cancer proteome unravel the cancer-specific regulation of glycolysis [ Cell Rep, 2021, 37(12):110137] PubMed: 34936872

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.