3-TYP

Catalog No.S8628 Batch:S862802

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Technical Data

Formula

C7H6N4
Molecular Weight 146.15 CAS No. 120241-79-4
Solubility (25°C)* In vitro DMSO 125 mg/mL (855.28 mM)
Ethanol 20 mg/mL (136.84 mM)
Water 2 mg/mL (13.68 mM)
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO Corn oil
6.25mg/ml Taking the 1 mL working solution as an example, add 50 μL of 125 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description 3-TYP (3-(1H-1,2,3-triazol-4-yl) pyridine) is a selective SIRT3 inhibitor which is selective for Sirt3 over Sirt1 and Sirt2. The IC50 values for SITR1, SIRT2, SIRT3 are 88 nM, 92 nM, 16 nM respectively.
Targets
SIRT3 [1]
(cell-free)		 SIRT1 [1]
(cell-free)		 SIRT2 [1]
(cell-free)
16 nM 88 nM 92 nM
In vitro Melatonin-induced increases in deacetylated-SOD2 expression and SOD2 activity are significantly attenuated by 3-TYP in HepG2 cells exposed to Cd (cadmium)[2].

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    HepG2 cells

  • Concentrations

    50 μM

  • Incubation Time

    12 h

  • Method

    Cells are randomly divided into the following treatment groups: 10 μM Cd; melatonin (1 μM)+10 μM Cd; 3-TYP (50 μM)+10 μM Cd; and 3-TYP (50 μM)+melatonin (1 μM)+10 μM Cd. cells are treated for 12 h. The role of the SIRT3-SOD2 pathway in hepatic cell protection after melatonin pretreatment is investigated.
Animal Study:

[3]
  • Animal Models

    Sprague-Dawley rats

  • Dosages

    5 mg/kg

  • Administration

    i.v.

Customer Product Validation

Data from [Data independently produced by , , CNS Neurosci Ther, 2018, doi:10.1111/cns.13053]

Selleck's 3-TYP has been cited by 33 publications

KSHV vIL-6 promotes SIRT3-induced deacetylation of SERBP1 to inhibit ferroptosis and enhance cellular transformation by inducing lipoyltransferase 2 mRNA degradation [ PLoS Pathog, 2024, 20(3):e1012082] PubMed: 38470932
Gravitational and mechanical forces drive mitochondrial translation [ bioRxiv, 2024, 10.1101/2023.01.18.524628] PubMed: none
Dynamics of Mitochondrial Proteome and Acetylome in Glioblastoma Cells with Contrasting Metabolic Phenotypes [ Preprint, 2024 , 2024011891] PubMed: none
Melatonin attenuates sepsis-induced acute kidney injury by promoting mitophagy through SIRT3-mediated TFAM deacetylation [ Autophagy, 2023, 1-15.] PubMed: 37651673
Exogenous NADPH exerts a positive inotropic effect and enhances energy metabolism via SIRT3 in pathological cardiac hypertrophy and heart failure [ EBioMedicine, 2023, 10.1016/j.ebiom.2023.104863] PubMed: 37950995
PDHA1 hyperacetylation-mediated lactate overproduction promotes sepsis-induced acute kidney injury via Fis1 lactylation [ Cell Death Dis, 2023, 14(7):457] PubMed: 37479690
Honokiol prevents chronic cerebral hypoperfusion induced astrocyte A1 polarization to alleviate neurotoxicity by targeting SIRT3-STAT3 axis [ Free Radic Biol Med, 2023, 202:62-75] PubMed: 36997099
Honokiol attenuates mitochondrial fission and cell apoptosis by activating Sirt3 in intracerebral hemorrhage [ Chin Med J (Engl), 2023, 136(6):719-731] PubMed: 36805606
HSP70 attenuates compression-induced apoptosis of nucleus pulposus cells by suppressing mitochondrial fission via upregulating the expression of SIRT3 [ Exp Mol Med, 2022, 10.1038/s12276-022-00745-9] PubMed: 35338257
Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells [ Dev Cell, 2022, 57(2):260-276.e9] PubMed: 35077680

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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