Dihydroartemisinin (DHA)

For research use only.

Catalog No.S2290 Synonyms: Dihydroqinghaosu, β-Dihydroartemisinin, Artenimol

16 publications

Dihydroartemisinin (DHA) Chemical Structure

CAS No. 71939-50-9

Dihydroartemisinin (DHA, Dihydroqinghaosu, β-Dihydroartemisinin, Artenimol) is a semi-synthetic derivative of artemisinin and isolated from the traditional Chinese herb Artemisia annua. Dihydroartemisinin induces autophagy and apoptosis by suppressing NF-κB activation.

Selleck's Dihydroartemisinin (DHA) has been cited by 16 publications

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  • (D) Western Blot analysis of TCTP in cell lysates of MDA cells after 24, 48 and 72 h of exposition to DHA. β-actin was used as loading control.

    Oncotarget, 2015, 6(7):5275-91.. Dihydroartemisinin (DHA) purchased from Selleck.

  • In the migration assays, the TCTP-positive cell lines NOZ, GBC-SD, and OCUG-1, and the TCTP-negative cell lines EH-GB-2 and SGC-996 were pre-treated with either vehicle or DHA (40 μM) for 2 days and then seeded in transwell plates for 24 h.

    J Exp Clin Cancer Res, 2017, 36(1):68. Dihydroartemisinin (DHA) purchased from Selleck.

  • Overexpression of GLUT1 inhibits cell death triggered by DHA. A549 and PC-9 cells were transfected with control (Ctr) and GLUT1 vector for 8 h respectively, and then treated with the indicated concentration of DHA for 48 h. (A) Cell lysates were subjected to western blot analysis with the indicated antibodies. (B) Cell growth inhibition activity was assessed by MTT. This experiment was repeated thrice. Columns, mean; bars, SD. ***, P < 0.001.

    PLoS One, 2015, 10(3):e0120426.. Dihydroartemisinin (DHA) purchased from Selleck.

  • H1975 and A549 cells were treated with increasing concentration of DHA for 1 day (left) or with 15 μM DHA for up to 24 h (right). Mcl-1 expression was examined by Western blotting.

    Biochem Pharmacol, 2018, 150:72-85. Dihydroartemisinin (DHA) purchased from Selleck.

  • Effects of various concentrations of DHA (5, 10, 20, 50 and 100 µM) on NCI-H1975 cell viability.

    Mol Med Rep, 2017, 16(3):3475-3481. Dihydroartemisinin (DHA) purchased from Selleck.

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Biological Activity

Description Dihydroartemisinin (DHA, Dihydroqinghaosu, β-Dihydroartemisinin, Artenimol) is a semi-synthetic derivative of artemisinin and isolated from the traditional Chinese herb Artemisia annua. Dihydroartemisinin induces autophagy and apoptosis by suppressing NF-κB activation.
NF-κB [4]
In vitro

Dihydroartemisinin (DHA) inhibits the growth of certain cancer cell lines and xenograft tumors such as leukemia, glioma, fibrosarcoma, and breast, cervical, ovarian, lung, oral and pancreatic cancer. DHA inhibits cell and tumor growth by modulating various tumor-suppressive pathways, such as inhibiting cell proliferation and inducing apoptosis through regulation of proliferation- and apoptosis-related proteins.DHA inhibits the proliferation and viability of cells in a dose-dependent manner and induces apoptosis.DHA-mediated cytotoxicity is tumor selective. The endoperoxide bridge of DHA is reportedly essential for its cytotoxicity because it reacts with intracellular ferrous iron to generate reactive oxygen species or carbon-centered radicals, leading to cytotoxicity[1].

Methods Test Index PMID
Western blot
p-STAT3 / p-JAK2 ; 

PubMed: 29434895     

The effects of dihydroartemisinin on phosphorylation of JAK2 and STAT3 were assessed using western blot analysis. 

MMP-2 / MMP-9 ; 

PubMed: 29472793     

The cells treated with dihydroartemisinin for 48 h were analyzed for the expression of MMP-2 and -9 using western blot analysis.

E-cadherin / N-cadherin / Vimentin / Twist / Slug / Snail ; 

PubMed: 29387451     

The expression of the EMT-related proteins were detected in A2780 or OVCAR3 cells after incubation with DHA for 24 h. (h) The expression of E-cadherin and twist in A2780 cell with knockdown of PDGFRα.

29434895 29472793 29387451

PubMed: 29387451     

Immunostaining of PDGFRα on the A2780 cell membrane after exposure to DHA for 24 h. The green signals represent PDGFRα staining, and the blue signals indicate the cell nuclei. Scale bar, 20 μm. 

Growth inhibition assay
Cell viability; 

PubMed: 29114376     

Cells incubated with different concentrations (0.1–200 μM) of dihydroartemisinin for 48 h and proliferation was assessed by MTT. Dihydroartemisinin reduced cell proliferation in EJ-138 and HTB-9 (a) cells in a dose-dependent manner.

In vivo

DHA significantly inhibited HCC cell growth in vitro and in vivo via inducing G2/M cell cycle arrest and apoptosis[2]. DHA has been shown in the rat whole embryo culture (WEC) to primarily affect primitive red blood cells (RBCs) causing subsequent tissue damage and dysmorphogenesis[3].


Cell Research:


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  • Cell lines: pancreatic cancer cell line BxPc3-RFP
  • Concentrations: 2.5, 10, 40, or 80 μM
  • Incubation Time: 24, 48, and 72 h
  • Method:

    BxPc3-RFP cells (3.5×104cells/well) were seeded in poly D-lysine-coated black, μClear 96-well plates with 0.2 ml medium. After 24 h, the cells were treated with dimethyl sulfoxide (DMSO) (control) or different concentrations (2.5, 10, 40, or 80 μM) of DHA dissolved in DMSO for 24, 48, and 72 h. At each time point, the fluorescence intensity emitted from cells was measured.

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Solubility (25°C)

In vitro DMSO 56 mg/mL warmed (196.94 mM)
Ethanol 11 mg/mL warmed (38.68 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 284.35


CAS No. 71939-50-9
Storage powder
in solvent
Synonyms Dihydroqinghaosu, β-Dihydroartemisinin, Artenimol
Smiles CC1CCC2C(C(OC3C24C1CCC(O3)(OO4)C)O)C

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03402789 Not yet recruiting Drug: Docosahexaenoic Acid|Drug: Placebo Oral Tablet Amblyopia Johns Hopkins University January 1 2022 Phase 1|Phase 2
NCT04965129 Not yet recruiting Drug: Placebo|Drug: Fish oil Lung Cancer Universidade Federal do Rio de Janeiro|Institutos Madrileño de Estudios Avanzados IMDEA|Oncoclínicas September 1 2021 Not Applicable

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID